The analysis of angiogenesis inhibition by PEDF using collagen-like triple helical peptides

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Although pigment epithelium-derived factor (PEDF) exhibits potent inhibitory activity against angiogenesis, its mechanism is unclear. Recently, our research group have identified the sequence on collagen which recognized PEDF. This sequence is also overlapped with heparan sulfate proteoglycan (HSPG)-recognizing sequence. In addition, PEDF interacts with not only collagen but also HSPG.We designed and synthesized collagen-like triple helical peptides which could specifically interact with PEDF or HSPG, and PEDF mutants which could specifically bind to collagen or HSPG. Furthermore, we optimized the condition of scratch assay as wound healing model to evaluate the effect of these compounds on angiogenesis.