The analysis of angiogenesis inhibition by PEDF using collagen-like triple helical peptides

使用类胶原三螺旋肽的 PEDF 抑制血管生成的分析

• 批准号: 24810026
• 负责人: MASUDA Ryo
• 金额: $ 1.91万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24810026/
• 关键词: PEDF; コラーゲン; ヘパラン硫酸; 血管新生; ヘパリン; HSPG; コラーゲンペプチド; 創傷治癒モデル; スクラッチアッセイ

Although pigment epithelium-derived factor (PEDF) exhibits potent inhibitory activity against angiogenesis, its mechanism is unclear. Recently, our research group have identified the sequence on collagen which recognized PEDF. This sequence is also overlapped with heparan sulfate proteoglycan (HSPG)-recognizing sequence. In addition, PEDF interacts with not only collagen but also HSPG.We designed and synthesized collagen-like triple helical peptides which could specifically interact with PEDF or HSPG, and PEDF mutants which could specifically bind to collagen or HSPG. Furthermore, we optimized the condition of scratch assay as wound healing model to evaluate the effect of these compounds on angiogenesis.

尽管色素上皮衍生因子(PEDF)具有很强的血管生成抑制活性，但其作用机制尚不清楚。最近，我们的研究小组已经确定了识别PEDF的胶原蛋白序列。该序列还与硫酸乙酰肝素蛋白多糖(HSPG)识别序列重叠。此外，PEDF不仅与胶原蛋白相互作用，还与HSPG相互作用。我们设计合成了能与PEDF或HSPG特异性结合的类胶原蛋白三螺旋多肽，以及能与胶原或HSPG特异性结合的PEDF突变体。此外，我们还对作为创伤愈合模型的划痕实验条件进行了优化，以评估这些化合物对血管生成的影响。

项目成果

所属研究室ホームページ

附属实验室主页