Understanding the contribution of T cells in infectious bursal disease virus immunopathogenesis and vaccine protection

了解 T 细胞在传染性法氏囊病病毒免疫发病机制和疫苗保护中的贡献

• 批准号: 453987525
• 负责人: Professorin Dr. Silke Rautenschlein, Ph.D.
• 金额: --
• 依托单位: Klinik für Geflügel
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/453987525?language=en
• 关键词: Understanding; contribution; cells; infectious; bursal

Infectious bursal disease virus (IBDV) is one of the most important immunosuppressive pathogens of poultry worldwide. While B lymphocytes are the main target cells of the virus, T lymphocytes are not only involved in controlling virus replication but may also interfere with the recovery of infected birds. The specific role of the different T cell subsets in infectious bursal disease (IBD) remains unclear, and therefore needs to be further elucidated to develop more efficient IBD-vaccination strategies. Our overall goal of this project is to provide a better understanding of the role of different T cell subsets in the phases of IBD. We will infect chickens that lack specific T cell subsets with IBDV strains of different virulence and compare them to infected non transgenic hatch-mates. Our specific aims are to determine the involvement of γδ T cells in IBDV pathogenesis and gut-associated immunity specifically in the acute phase of infection. Furthermore, we want to identify the involvement of αβ T cells in IBDV clearance and protection. To assess the role of regulatory T cells in the recovery process, we will focus mainly on the post-acute phase of the disease. In addition, we aim to identify T cell epitopes, as possible targets for future vaccine candidates. Our Work Package 1 (WP1) is part of a collaborative approach unravelling the avian immune response, and will be tightly embedded in the context of the research unit “ImmunoChick”, FOR5130. The individual projects will benefit from the collaborations among each other and provide a better insight into the immune system of chickens in relation to important pathogens.

传染性法氏囊病病毒(IBDV)是世界范围内最重要的禽类免疫抑制病原体之一。虽然B淋巴细胞是病毒的主要靶细胞，但T淋巴细胞不仅参与控制病毒复制，还可能干扰受感染禽类的恢复。不同T细胞亚群在传染性法氏囊病(IBD)中的具体作用尚不清楚，因此需要进一步阐明，以开发更有效的IBD疫苗接种策略。我们这个项目的总体目标是更好地了解不同T细胞亚群在IBD各阶段中的作用。我们将用不同毒力的IBDV毒株感染缺乏特定T细胞亚群的鸡，并将它们与感染的非转基因孵化鸡进行比较。我们的具体目的是确定γδT细胞在IBDV致病机制和肠道相关免疫中的作用，特别是在感染的急性期。此外，我们希望确定αβT细胞在清除和保护IBDV中的作用。为了评估调节性T细胞在康复过程中的作用，我们将主要关注疾病的急性后阶段。此外，我们的目标是确定T细胞表位，作为未来候选疫苗的可能靶点。我们的工作包1(WP1)是揭开鸟类免疫反应的合作方法的一部分，并将紧密嵌入研究单位“免疫鸡”的背景下，FOR5130。这些单独的项目将从彼此的合作中受益，并提供对鸡与重要病原体相关的免疫系统的更好了解。