Transcriptome analysis of phenotypic plasticity in the Hokkaido salamander

两栖动物显示灵活的表型取决于环境条件。北海道蝾螈的幼体，Hynobius retardatus，表现出两种不同的变体，“攻击变体”和“防御变体”，作为一种诱导的表型反应猎物和捕食者，分别。他们的猎物，蛙pirica蝌蚪的存在，诱导宽头攻击变形。捕食性的黑黄拟杆菌（Aeshna nigroflava）的存在诱导了一种防御形态，其特征是扩大的外鳃和高尾。然而，这些表型变化的分子机制尚未阐明。为了揭示这些诱导塑料变形的分子机制的景观，我们进行了从头转录组分析的北海道蝾螈幼虫。我们从取样的组织中提取RNA并测序。然后，我们比较处理样品的获得的读段和重叠群，并鉴定差异表达的基因。

Amphibians display flexible phenotypes depending on environmental conditions. Larvae of the Hokkaido salamander, Hynobius retardatus, exhibit two distinct morphs, "attack morphs" and "defense morphs", as an inducible phenotypic response to prey and predators, respectively. The presence of their prey, Rana pirica tadpoles, induces a broad-headed attack morph. The presence of predatory dragonfly nymphs (Aeshna nigroflava) induces a defense morph, characterized by enlarged external gills and a high tail. However, the molecular mechanisms underlying these phenotypic changes have yet to be elucidated. To reveal the landscape of molecular mechanisms underlying these inducible plastic morphs, we carried out a de novo transcriptome analysis of Hokkaido salamander larvae. We extracted and sequenced RNAs from the sampled tissues. Then, we compared the obtained reads and contigs of the treatment samples and identified differentially expressed genes.