Structural and functional analysis of a novel iron-sulpfur protein involved in the intracellular assembly of light-harvesting antenna

在本研究中，我们重点研究了超分子复合物在细胞内组装的分子机制。我们特别研究了该蛋白的结构和功能，该蛋白参与了蓝藻光收集天线复合物在类囊体膜上的组装。我们构建了蓝藻Ycf34表达质粒并纯化了这些蛋白。我们发现表达的Ycf34在蛋白中含有铁硫簇。突变分析表明，Ycf34的五个半胱氨酸残基中的每一个都参与了蛋白质中铁硫簇的保持。

In this study, we focused on the molecular mechanism of the intracellular assembly of supramolecular complex. Especially, we studied the structure and function of the protein, involved in the assembly of cyanobacterial light harvesting antennae complex on the thylakoid membrane. We constructed cyanobacterial Ycf34 expression plasmids and purified those proteins. We found the expressed Ycf34 holds iron-sulfur cluster in the protein. The mutation analysis indicated that each of five cysteine residues of Ycf34 involved in the holding of iron-sulfur cluster in the protein.