Structural studies on mechanism of amino acid exporting system in Corynebacterium glutamicum

谷氨酸棒杆菌是一种用于谷氨酸发酵的细菌。近年来发现了一种机械敏感性通道NCgl1221，它负责谷氨酸的输出，并且在谷氨酸发酵中起重要作用。阐明C.我们构建了NCgl1221在大肠杆菌中的表达系统，并成功地获得了纯化的NCgl1221。结晶筛选后，在几种条件下出现蛋白质晶体。在优化的晶化条件下得到的晶体，虽然分辨率较差，但仍能观察到较好的衍射图。

Corynebacterium glutamicum is a bacterium for glutamate fermentation. Recently, the mechanosensitive channel, NCgl1221 is uncovered to be responsible for the export of glutamate and is important for the glutamate fermentation. To elucidate the mechanism of glutamate exporting system in C. glutamicum structurally, we performed the crystallographic analysis of NCgl1221We constructed the expression system of NCgl1221 in Escherichia coli and we could successfully obtain purified NCgl1221. After crystallization screening, protein crystals appeared in several conditions. Although the resolution was poore, we could observe the good diffraction patterns from the crystals obtained in the optimized crystallization condition.