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Structural studies on mechanism of amino acid exporting system in Corynebacterium glutamicum

谷氨酸棒杆菌氨基酸输出系统机制的结构研究

基本信息

批准号：
24880011
负责人：
YOSHIDA Ayako
金额：
$ 1.91万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Research Activity Start-up
财政年份：
2012
资助国家：
日本
起止时间：
2012-08-31 至 2014-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24880011/
关键词：
メカノセンシティブチャネルグルタミン酸発酵コリネバクテリウム　グルタミカム膜タンパク質 X線結晶構造解析グルタミン酸醗酵コリネバクテリウム応用微生物学アミノ酸発酵タンパク質X線結晶構造解析

项目摘要

Corynebacterium glutamicum is a bacterium for glutamate fermentation. Recently, the mechanosensitive channel, NCgl1221 is uncovered to be responsible for the export of glutamate and is important for the glutamate fermentation. To elucidate the mechanism of glutamate exporting system in C. glutamicum structurally, we performed the crystallographic analysis of NCgl1221We constructed the expression system of NCgl1221 in Escherichia coli and we could successfully obtain purified NCgl1221. After crystallization screening, protein crystals appeared in several conditions. Although the resolution was poore, we could observe the good diffraction patterns from the crystals obtained in the optimized crystallization condition.

谷氨酸棒杆菌是一种用于谷氨酸发酵的细菌。最近，机械敏感通道NCgl1221被发现负责谷氨酸的输出，并且对于谷氨酸发酵很重要。为了从结构上阐明谷氨酸棒杆菌谷氨酸输出系统的机制，我们对NCgl1221进行了晶体学分析，构建了NCgl1221在大肠杆菌中的表达系统，并成功获得了纯化的NCgl1221。经过结晶筛选后，蛋白质晶体在几种条件下出现。尽管分辨率较差，但我们可以从优化结晶条件下获得的晶体中观察到良好的衍射图样。