Elucidation of the nature of leukemic stem cells in murine erythroleukemia model

我们将JAK2V617F逆转录病毒导入p53野生型和基因敲除小鼠的骨髓细胞，并将其移植到受照射的受者体内。如前所述，JAK2V617F在野生型造血细胞中的表达会导致真性红细胞增多症(PV)。相反，在没有p53的情况下，受者在前一个PV期之后发展为致命性白血病，特征是发育不良的红系祖细胞扩大。这些白血病细胞是可连续移植的。原始白血病细胞和红系祖细胞都具有启动白血病的能力，而髓系细胞不能重现这种疾病。

We retrovirally transduced JAK2V617F into bone marrow cells from p53 wild-type and knockout mice and transplanted them into irradiated recipients. As previously shown, JAK2V617F expression in wild-type hematopoietic cells causes polycythemia vera (PV). In contrast, in the absence of p53, recipients developed fatal leukemia after the preceding PV phase, characterized by expansion of dysplastic erythroid progenitors. These leukemic cells are serially transplantable. Both primitive leukemia cells and erythroid progenitors possess leukemia-initiating capacity, whereas myeloid populations cannot recapitulate the disease.