Functional analysis of human profilaggrin C-terminus region: Identification of molecular mechanism in profilaggrin processing

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Filaggrin monomers are produced through profilaggrin (FLG) processing, and they are considered to be crucial in epidermal barrier function. Recently, lack of FLG C-terminus region in filaggrin gene mutation carriers was shown to be associated with significant reduction in filaggrin monomer production. To this end, we sought to evaluate the function of FLG C-terminus region in FLG processing. 1) Three types of C-terminus expression vectors were constructed, and these constructs were transfected to COS-7 cells followed by RT-PCR and Western blot analysis. 2) By pull-down assay using FLG C-terminus protein, molecules with interacting potential were searched. As result, 1) deletion of FLG C-terminus region did not alter the FLG mRNA/protein expression, suggesting that FLG C-terminus region is possibly involved in the processing of FLG, and not with the production of FLG itself. 2) Candidate proteins that interact with FLG C-terminus region were identified, such as galectin-7, etc.