Mechanism of activation of ryanodine receptor by diamide insecticide

氟苯二胺对鳞翅目昆虫具有选择性杀虫活性。Ryanodine受体(RyR)钙释放通道是氟苯二胺的主要靶点。然而，氟苯二胺的物种特异性作用的分子机制尚不清楚。为了鉴定RyR中的氟苯二胺结合区，我们使用了新的亲和探针和LC/MS/MS分析。这些结果表明，RyR的氟苯二胺反应需要多样性区域1。我们的发现为理解氟苯二胺的物种选择作用提供了重要的信息。

Flubendiamide shows selective insecticidal activity against lepidopterous insects. The ryanodine receptor (RyR) Ca2+ release channel is a primary target of flubendiamide. However, the molecular mechanisms underlying the species-specific action of flubendiamide are unclear. To identify the flubendiamide-binding region within RyR, we used new affinity probe and LC/MS/MS analysis. The results suggest that the flubendiamide response of RyR requires the diversity region 1. Our findings provide important information for understanding the species-selective effects of flubendiamide.