Study for the development of new fracture treatment by the adhesive technology evolution and molecular engineering

骨形态发生蛋白家族蛋白因其能在成体内稳态诱导骨形态发生以及在发育早期诱导表皮和背神经管分化而被广泛应用于生物学研究和医学应用。利用生长因子通过骨移植刺激骨形态发生是再生医学中一种很有前途的方法。我们创造了一个由骨形态蛋白-4和纤维连接蛋白的胶原结合域多肽组成的融合蛋白。该融合蛋白(CBD-BMP4)由杆状病毒表达系统产生，具有很强的胶原结合活性。本研究的目的是检测这种融合蛋白修复骨缺损的效果。

BMP family proteins are widely used for biological research and medical application because of their ability to induce bone morphogenesis at adult homeostasis and differentiation of epidermis and dorsal neural tube at early development. Utilizing growth factors that stimulate bone morphogenesis with a bone implant is a promising approach in regenerative medicine. We create a fusion protein consisting of bone morphologic protein-4 and a collagen-binding domain polypeptide of fibronectin. This fusion protein (CBD-BMP4), produced by a baculovirus expression system, exhibited much strong collagen binding activity. The aim of this study is to examine the effect of this fusion protein for repair of bone defect.