Functional analysis of candidate pseudopodial molecules of breast cancer identified by a novel application of laser proteomics

通过激光蛋白质组学的新应用鉴定的乳腺癌候选伪足分子的功能分析

• 批准号: 24890137
• 负责人: MIMAE TAKAHIRO
• 金额: $ 1.91万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890137/
• 关键词: 癌浸潤; プロテオミクス

The cancer invasion has a potential to threaten the lives of patients. Therefore, the regulation of invasion will lead to conquest of cancer. In the present study, we attempted to disclose the mechanism of invadopodia or pseudopodia microprocesses formation which are known to be involved in cell motility or cancer cell invasion. We developed a novel application for precise isolation of invadopodia or pseudopodia. The protein fraction of pseudopodia was collected by this novel application, then, the candidate pseudopodial molecules were identified. In addition, the functional analysis of some candidate molecules was performed. The outcomes of the study was published in papers and annual meeting of AACR2013. The present study will provide further understandings of cancer research.

癌症侵袭有可能威胁患者的生命。因此，调节入侵将导致癌症的征服。在本研究中，我们试图揭示侵袭伪足或伪足微突起的形成机制，这些微突起已知与细胞运动或癌细胞侵袭有关。我们开发了一种新的应用程序，用于精确分离侵入伪足或伪足。利用该方法收集伪足的蛋白质组分，并对候选伪足分子进行鉴定。此外，对部分候选分子进行了功能分析。研究结果发表在AACR 2013年度会议和论文中。本研究将有助于进一步了解癌症研究。

项目成果

Novel application for pseudopodial proteomics using excimer laserablation and two-dimensional difference gel electrophoresis

使用准分子激光烧蚀和二维差异凝胶电泳进行伪足蛋白质组学的新应用

• 发表时间: 2013
• 作者: Takahiro Mimae;Akihiko Ito;Man Hagiyama;Tadashi Kondo;Morihito Okada
• 通讯作者: Morihito Okada

Novel application for pseudopodial proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis

准分子激光烧蚀和二维差异凝胶电泳在伪足蛋白质组学中的新应用

• 发表时间: 2013
• 作者: Takahiro Mimae;Akihiko Ito;Man Hagiyama;Tadashi Kondo;Morihito Okada
• 通讯作者: Morihito Okada

Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis

• DOI: 10.1038/labinvest.2012.98
• 发表时间: 2012-09-01
• 期刊: LABORATORY INVESTIGATION
• 影响因子: 5
• 作者: Ito, Akihiko;Mimae, Takahiro;Kondo, Tadashi
• 通讯作者: Kondo, Tadashi

α-Parvin, a pseudopodial constituent, promotes cell motility and is associated with lymph node metastasis of lobular breast carcinoma

• DOI: 10.1007/s10549-014-2859-0
• 发表时间: 2014-02-01
• 期刊: BREAST CANCER RESEARCH AND TREATMENT
• 影响因子: 3.8
• 作者: Ito, Masaoki;Hagiyama, Man;Ito, Akihiko
• 通讯作者: Ito, Akihiko