Analysis of relationship between periodontal tissue para-inflammation induced neuroinflammation and phagocytic activity regulation by circadian cloclk system

牙周组织副炎症诱导的神经炎症与生物钟系统吞噬细胞活性调节关系分析

• 批准号: 24890161
• 负责人: OKADA Ryo
• 金额: $ 1.66万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890161/
• 关键词: 歯学; 歯周パラインフラメーション; 脳炎症; 食細胞; 体内時計

To elucidate the mechanism of cognitive impairment by chronic inflammatory periodontal disease, also known as para-inflammation disease, we developed animal models of periodontal disease. After the application of P. gingivalis (PG) to periodontal tissues, proinflammatory cytokines were expressed at the periodontal tissue and cerebral cortex. and intraperitoneal administration of PG caused cognitive impairment.Microglia involved in cognitive impairment by producing proinflammatory cytokines under neuroinflammatory conditions. And recent studies showed that the abilities of microglia showed circadian rhythm. After the application of PG, proinflammatory cytokine mRNA expression levels in brain microglia and periodontal tissue macrophages showed circadian rhythm. These results suggested that circadian rhythm of proinflammatory cytokine gene expressions by periodontal tissue and brain phagocytes may affect periodontal tissue inflammation and cognitive impairment progression.

为了阐明慢性炎症性牙周病（也称为炎症旁疾病）导致认知障碍的机制，我们建立了牙周病动物模型。牙龈卟啉单胞菌（PG）作用于牙周组织后，牙周组织和大脑皮质表达促炎细胞因子。腹腔注射PG可引起认知功能障碍，小胶质细胞通过在神经炎症条件下产生促炎细胞因子参与认知功能障碍。近年来的研究表明，小胶质细胞的功能具有昼夜节律性。应用PG后，脑小胶质细胞和牙周组织巨噬细胞中促炎细胞因子mRNA表达水平呈昼夜节律。这些结果表明，牙周组织和脑吞噬细胞的促炎细胞因子基因表达的昼夜节律可能会影响牙周组织炎症和认知障碍的进展。

项目成果

Batten病モデルマウスにおける脳ペリサイトの酸化ストレス障害と血液脳関門の破綻

巴顿病模型小鼠脑周细胞的氧化应激损伤和血脑屏障的破坏

• 发表时间: 2013
• 作者: 岡田 亮;武 洲;倪 軍軍;中西 博
• 通讯作者: 中西 博

The intrinsic microglial molecular clock controls synaptic strength via the circadian expression of cathepsin S.

• DOI: 10.1038/srep02744
• 发表时间: 2013-09-25
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Hayashi, Yoshinori;Koyanagi, Satoru;Kusunose, Naoki;Okada, Ryo;Wu, Zhou;Tozaki-Saitoh, Hidetoshi;Ukai, Kiyoharu;Kohsaka, Shinichi;Inoue, Kazuhide;Ohdo, Shigehiro;Nakanishi, Hiroshi
• 通讯作者: Nakanishi, Hiroshi

Diurnal spatial reorganization of Microglial Processes through the rhythmic expression of P2Y12 receptors

通过 P2Y12 受体的节律表达进行小胶质细胞过程的昼夜空间重组

• 发表时间: 2013
• 期刊: Journal of Neurological Disorders
• 作者: Yoshinori Hayashi;Satoru Koyamagi;Naoki Kusunose;Fumiko Takayama;Ryo Okada;Zhou Wu;Shigehiro Ohdo;Hiroshi Nakanishi
• 通讯作者: Hiroshi Nakanishi

カテプシンD欠損マウスの脳病変における脳ペリサイト消失と免疫細胞の脳内浸潤の関与

组织蛋白酶D缺陷小鼠脑周细胞消失和免疫细胞脑内浸润参与脑损伤

• 发表时间: 2013
• 作者: 岡田 亮;武 洲;倪 軍軍;中西 博
• 通讯作者: 中西 博

Apoptosis-dependent Externalization and Involvement in Apoptotic Cell Clearance of DmCaBP1, an Endoplasmic Reticulum Protein of Drosophila

• DOI: 10.1074/jbc.m111.277921
• 发表时间: 2012-01-27
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Okada, Ryo;Nagaosa, Kaz;Nakanishi, Yoshinobu
• 通讯作者: Nakanishi, Yoshinobu