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The study of embryonic hemoglobin re-expression in adult mice.

成年小鼠胚胎血红蛋白重新表达的研究。

基本信息

批准号：
24890237
负责人：
OTSUKA Hirotada
金额：
$ 1.91万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Research Activity Start-up
财政年份：
2012
资助国家：
日本
起止时间：
2012-08-31 至 2014-03-31
项目状态：
已结题

项目摘要

Mammal hematopoiesis occurs two distinct waves, kwon as a primitive and definitive; and to express the hemoglobin of different types are known. However, there is no possibility that the hematopoietic system of these two simultaneous presence in the adult.We established an experimental system to re-express the embryonic hemoglobin in adult mice by inducing the critical anemia treated with nitrogen containing bisphosphonate and phenylhydrazine, and we detected the cell, expressing at the same time the erythroid marker and embryonic hemoglobin in the liver. We also confirmed that the hemoglobin subunites of adult and embryo types were simultaneously present in the peripheral blood. In addition, it was possible to confirm the factors, which regulates the expression and switching of hemoglobin. These results support that understanding the mechanism of hemoglobin expression and switching during development.

哺乳动物的造血发生了两次截然不同的浪潮，权氏作为一种原始的和确定的；而表达不同类型的血红蛋白是已知的。然而，这两种造血系统在成年后不可能同时存在。我们建立了一种实验体系，通过含氮双膦酸联苯肼处理的临界贫血诱导成年小鼠胚胎血红蛋白的重新表达，并对细胞进行检测，同时在肝脏中表达红系标志和胚胎血红蛋白。我们还证实，成年型和胚胎型的血红蛋白亚基同时存在于外周血中。此外，还有可能确认调节血红蛋白表达和转换的因素。这些结果支持理解发育过程中血红蛋白表达和转换的机制。