RNA-guided Biotin Ligases for the Analysis of Protein-Oligonucleotide Interactions

RNA 引导的生物素连接用于分析蛋白质-寡核苷酸相互作用

• 批准号: 455100081
• 负责人: Professor Dr. Thorsten Stafforst
• 金额: --
• 依托单位: Interfakultäres Institut für Biochemie (IFIB)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/455100081?language=en
• 关键词: RNA; guided; Biotin; Ligases; Analysis

The aim of this project is the development and application of biotin ligases for the RNA-centric analysis of the interactome of specific RNAs with proteins. Subproject A) analyzes the interactions of proteins with chemically modified, therapeutically relevant antisense oligonucleotides inside the living cell. Subproject B) investigates the protein interactome of specific endogenous transcripts. For this, biotin ligases are being developed that can be specifically addressed by means of RNA conjugation, and the protein/RNA interactome is analyzed by LC-HR-MS/MS.

本项目的目的是开发和应用生物素连接酶，以RNA为中心分析特定RNA与蛋白质的相互作用组。子项目A）分析活细胞内蛋白质与化学修饰的、治疗相关的反义寡核苷酸的相互作用。子项目B）研究特定内源性转录物的蛋白质相互作用组。为此，正在开发生物素连接酶，其可以通过RNA缀合特异性地解决，并且通过LC-HR-MS/MS分析蛋白质/RNA相互作用组。