Functional Analysis of the NMDA-recepter-APC-DLG-Complex.

NMDA-受体-APC-DLG-复合物的功能分析。

• 批准号: 11470039
• 负责人: AKIYAMA Testu
• 金额: $ 9.28万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470039/
• 关键词: NMDA receptor; Tumor suppressor; APC; PSD-95; SPAL; synapse; post synaptic density; learning; 神経; 海馬; NMAD受容体; DLG

The synaptic protein PSD95/SAP90 interacts with ion channels such as the N-methyl-D-aspartate-receptor (NMDA-R) via its PDZ domain, and is involved in their clustering. Moreover, it interacts with signaling molecules and plays an important role in coupling NMDA-R to pathways that control synaptic plasticity and learning. We found that PSD-95 is associated with the adenomatous polyposis coli (APC) tumor suppressor protein, Rap1-specific GAP (SPAL), LGN and tyrosine kinase Fyn. Our findings raise the possibility that these molecules are involved in the regulation of NMDA-R clustering and/or NMDA-R-mediated signal transduction.

突触蛋白PSD95/SAP90通过其PDZ结构域与N-甲基-D-天冬氨酸受体(NMDA-R)等离子通道相互作用，并参与它们的聚集。此外，它还与信号分子相互作用，并在将NMDA-R偶联到控制突触可塑性和学习的途径中发挥重要作用。我们发现PSD-95与结肠腺瘤性息肉病(APC)肿瘤抑制蛋白、RAP1特异性GAP(SPAL)、LGN和酪氨酸激酶Fyn相关。我们的发现提出了这些分子参与调控NMDA-R聚集和/或NMDA-R介导的信号转导的可能性。

项目成果

T.Ishidate: "The APC-hDLG Complex Negatively Regulates Cell Cycle Progression from the G0/G1 to SPhase."Oncogene. 19. 356-372 (1999)

T.Ishidate：“APC-hDLG 复合物负向调节从 G0/G1 到 SPhase 的细胞周期进展。”癌基因。

Y.Dobashi: "APC protein is required for initiation of neuronal differentiation in rat pheochromocytoma PC12 cells."Biochem.Biophys.Res.Commun.. 279. 685-691 (2000)

Y.Dobashi：“大鼠嗜铬细胞瘤 PC12 细胞中神经元分化的启动需要 APC 蛋白。”Biochem.Biophys.Res.Commun.. 279. 685-691 (2000)

Kakinuma,N.: "APC is Colocalized with β-Catenin and hDLG in the Henle's Loop of the Mouse Kidney."Acta Histochem.Cytochem.. (in press).

Kakinuma, N.：“APC 与 β-Catenin 和 hDLG 共定位于小鼠肾脏的亨利环中。”Acta Histochem.Cytochem..（出版中）。

K.Haraguchi, K.Satoh, H.Yanai, F.Hamada, M.Kawabuchi and T.Akiyama: "The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase."Genes Cells. 5. 905-911 (2000)

K.Haraguchi、K.Satoh、H.Yanai、F.Hamada、M.Kawabuchi 和 T.Akiyama：“hDLG 相关蛋白 DAP 与动力蛋白轻链和神经元一氧化氮合酶相互作用。”Genes Cells。

C.R,Badal: "Localization of the GAP family SPAL in the Rat Esophagus and Heart."Med.Elcetron Microse.. 32. 20-24 (1999)

C.R,Badal：“GAP 家族 SPAL 在大鼠食道和心脏中的定位。”Med.Elcetron Microse.. 32. 20-24 (1999)