ANALYSIS OF MICROCHIMERISM BY TRANSPLANT PRETREATMENT OF ALLOANTIGEN OR XENOANTIGEN IN LIVER AND SMALL BOWEL TRANSPLANTATION

1997-2000年间，我们有三个执行计划。在微嵌合诱导研究中，将供体脾细胞与FK506联合给药，短时间围给药。减少同种异体移植排斥反应，延长大鼠小肠移植模型存活时间。在无毒HSP 70诱导剂香叶酮（geranylgeranylacetone， GGA）抗凋亡作用的研究中，GGA对暴露于过氧化氢（H202）或乙醇的肝细胞诱导HSP 70，并发挥抗凋亡作用。这些结果表明GGA可能对肝损伤的治疗有潜在的益处。接下来，我们研究了GGA对大鼠95%肝切除术后急性肝损伤的影响。25只载药大鼠在95%肝切除术后60小时内全部死亡，而25只GGA大鼠中有10只存活。GGA显著抑制转氨酶和IL-6的释放，完全抑制TNF-α的产生。目前正在缺血性肝移植模型中进行检查。本研究探讨了FK506和OKY046 （Tromboxane Synthase I inhibitor）对大鼠原位肝移植非心脏跳动供体（NHBD）热缺血再灌注损伤的影响。FK506和OKY046联合组14天存活率为100%，FK506组为83%，OKY046组为83%，而对照组仅为33%。FK506和OKY046联合组、FK506组和OKY046组再灌注后血清AST、ALT、LDH、il - 6、TNF-α浓度均显著升高。

We had three execution plan trough the 1997-2000 year.1. In regard to microchimerism induction study, combined administration of the donor splenocytes and FK506 with the short periadministration. reduced allograft rejection and prolonged survival in the rat model of small bowel transplantation.2. In regard to studies about anti-apoptosis action of non-toxic HSP 70 inducer, geranylgeranylacetone (GGA), GGA administration induced HSP 70 on hepatocytes exposed to hydrogen peroxide (H202) or ethanol and exerted anti-apoptosis action. These results suggest that GGA may have a potential benefit for the treatment of liver injury. Next, we examined the effects of GGA on acute hepatic injury after 95% hepatectomy in rats. All of 25 rats with vehicle died within 60 hours after 95% hepatictomy, while 10 Of 25 rats with GGA survived. And GGA significantly suppressed the release of transaminases and IL-6 and completely inhibited TNF-α production. The examination is being carried out in the ischemic liver transplantation model at present.3. In this study, we examined the effect of FK506 and OKY046 (Tromboxane Synthase I nhibitor) against warm ischemia-reperfusion injury on non-heart-beating donor (NHBD) in orthotopic liver transplantation in rats. The 14 days survival rate in FK506 and OKY046 combined group was 100%, 83% in FK506 group, 83% in OKY046 group, compared to only 33% in control group. And serum AST, ALT, LDH, IL6 and TNF-α concentration after reperfusion were significantly improved in FK506 and OKY046 combined group, in FK506 group and in OKY046 group.