Immuno-histochemical and microbiological study on the relationships between microcirculatory system and immuno-defence system in the dental pulp.

为了阐明牙髓微循环系统和免疫防御系统之间的关系，我们对人的血液和淋巴管进行了免疫组织化学和微生物学检测。结果如下：1)正常组织中淋巴管仅表达PECAM-1，炎症组织中表达PECAM-1、ICAM-1、ICAM-3、VCAM-1。1)颌下淋巴结淋巴管表达PECAM-1、ICAM-1、VCAM-1；舌部淋巴管表达PECAM-1、ICAM-1、ELAM-1或PECAM-1、ICAM-1。在正常牙髓血管中，PECAM-1强表达，而MHC-II、ELAM-1、ICAM-1.7弱表达，PFCAM-1、MHC-II、ELAM-1、ICAM-1在炎症牙髓血管中有强表达。1)在正常牙髓和炎症牙髓的淋巴管中，PECAM-1呈强阳性表达，ICAM-1呈弱阳性表达。1)PECAM-1仅在淋巴管内皮细胞的管腔表面表达，而在淋巴管内皮细胞的管腔表面和管腔表面均有表达。

To clarify the relationships between microcirculatory system and immuno-defence system in the dental pulp, we examined human blood and lymphatic vessels immuno-histochemically and microbiologically. The results are summarized as follows :1)In general, lymphatic vessels in healthy tissue express PECAM-1 only, but in inflamed tissue, they express PECAM-1, ICAM-1, ICAM-3, and VCAM-1.2) In the submandibular lymph node, lymphatic vessels express PECAM-1 and ICAM-1.3) In the tongue, lymphatic capillaries close to oral epithelitum express PECAM-1, ICAM-1, and ELAM-1, or PECAM-1 and ICAM-1. However, collecting lymphatic vessels express PECAM-1 only.4) Mediation of inflammatory cytokines induce strong expression of PECAM-1, and expression of ICAM-1 on the lymphatic vessels.5) Some oral microbial associates induce expression of leukocyte adhesion molecules and production of cytokines in the vascular endothelial cells.6) In blood vessels of healthy dental pulp, there are strong expression of PECAM-1 and weak expression of MHC class II, ELAM-1, and ICAM-1.7) In blood vessels of inflamed dental pulp, there are strong expression of PFCAM-1, MHC class II, ELAM-1, ICAM-1, ICAM-3, and VCAM-1.8) In lymphatic vessels of healthy and inflamed dental pulp, there are strong expression of PECAM-1 and weak expression of ICAM-1.9) PECAM-1 expression of the blood vessels was observed only on the luminal surface of the endothelium, but in the lymphatic vessels, it was found both on the luminal and abluminal surfaces of the endothelium.10) Expression of desmoplakin is observed only in the lymphatic vessels, but not in the blood vessels, indicating that anti-desmoplakin antibody is useful to discriminate lymphatic vessels from blood vessels.