Immuno-histochemical and microbiological study on the relationships between microcirculatory system and immuno-defence system in the dental pulp.

为了阐明牙髓中微循环系统与免疫防御系统的关系，我们对人血液和淋巴管进行了免疫组织化学和微生物学检测。结果表明：1)一般来说，健康组织的淋巴管只表达PECAM-1，但在炎症组织中，它们表达PECAM-1、ICAM-1、ICAM-3和VCAM-1.2)在下颌下淋巴结，淋巴管表达PECAM-1和ICAM-1.3)在舌上，靠近口腔上皮的淋巴管毛细血管表达PECAM-1、ICAM-1和ELAM-1，或PECAM-1和ICAM-1。然而，收集淋巴管只表达PECAM-1。4)炎症细胞因子的介导可诱导PECAM-1和ICAM-1在淋巴管上的强表达。5)一些口腔微生物伴生物诱导血管内皮细胞中白细胞粘附分子的表达和细胞因子的产生。6)在健康牙髓血管中，PECAM-1表达较强，MHCⅱ类、ELAM-1、ICAM-1.7表达较弱。在炎症牙髓血管中，PFCAM-1、MHCⅱ类、ELAM-1、ICAM-1、ICAM-3、VCAM-1.8表达较强。PECAM-1表达强烈，icam -1表达微弱（1.9）。PECAM-1在血管中仅在内皮的管腔表面表达，而在淋巴管中，内皮的管腔和管腔表面均有表达。10) desmoplakin仅在淋巴管中表达，而在血管中未见表达，提示抗desmoplakin抗体可用于区分淋巴管和血管。

To clarify the relationships between microcirculatory system and immuno-defence system in the dental pulp, we examined human blood and lymphatic vessels immuno-histochemically and microbiologically. The results are summarized as follows :1)In general, lymphatic vessels in healthy tissue express PECAM-1 only, but in inflamed tissue, they express PECAM-1, ICAM-1, ICAM-3, and VCAM-1.2) In the submandibular lymph node, lymphatic vessels express PECAM-1 and ICAM-1.3) In the tongue, lymphatic capillaries close to oral epithelitum express PECAM-1, ICAM-1, and ELAM-1, or PECAM-1 and ICAM-1. However, collecting lymphatic vessels express PECAM-1 only.4) Mediation of inflammatory cytokines induce strong expression of PECAM-1, and expression of ICAM-1 on the lymphatic vessels.5) Some oral microbial associates induce expression of leukocyte adhesion molecules and production of cytokines in the vascular endothelial cells.6) In blood vessels of healthy dental pulp, there are strong expression of PECAM-1 and weak expression of MHC class II, ELAM-1, and ICAM-1.7) In blood vessels of inflamed dental pulp, there are strong expression of PFCAM-1, MHC class II, ELAM-1, ICAM-1, ICAM-3, and VCAM-1.8) In lymphatic vessels of healthy and inflamed dental pulp, there are strong expression of PECAM-1 and weak expression of ICAM-1.9) PECAM-1 expression of the blood vessels was observed only on the luminal surface of the endothelium, but in the lymphatic vessels, it was found both on the luminal and abluminal surfaces of the endothelium.10) Expression of desmoplakin is observed only in the lymphatic vessels, but not in the blood vessels, indicating that anti-desmoplakin antibody is useful to discriminate lymphatic vessels from blood vessels.