Source and dynamics of dissolved and particulate proteins in the hydrosphere.

溶解态和颗粒态有机质的来源和动力学是深入了解全球碳循环的关键问题。研究结果表明，水圈中溶解的和颗粒状有机物的无生命池中存在特定的蛋白质分子。在马恩环境中，不到30种蛋白质在不同的海洋区域被检测到溶解相。其中一种溶解蛋白被鉴定为存在于革兰氏阴性菌铜绿假单胞菌外膜中的一种称为孔蛋白的特定类型蛋白的同源物。通过免疫化学检测，其中一种蛋白也被鉴定为另一种类型的猪。在淡水环境中，我们也检测到了一些溶解相的蛋白分子，其中一种蛋白被鉴定为另一种来自革兰氏阴性菌百日咳杆菌的孔蛋白;根据分子量分布的纵向和纵向差异，我们将海洋沃茨中的颗粒蛋白分为两组：背景蛋白和特异蛋白。通过N-末端氨基酸序列分析，确定了浮游植物叶绿体膜中存在的热休克蛋白70（Heat shock protein 70）。这些蛋白质的发现为水圈中无生命有机库的来源和动力学以及全球碳循环提供了宝贵的信息。

Source and dynamics of dissoloved and particulate organic matter is key question for better understanding of global carbon cycle. Results of this research indicated that there are specific protein molecules in the inanimate pools of dissolved and particulate organic matter in the hydrosphere. In marne environments, less than 30 proteins were detected in dissolved phase over the various oceanic areas. One of dissolved protein was identified as a homologue of a specific type of protein called porin, exists in the outer-membrane of the Gram-negative bacterium, Pseudomona aeruginosa. By means of immunochemical detection, one of the other protein also identified as another type of porine. In freshwater environment, we also detected several protein molecules in dissolved phase and one of the dissolved protein was identified as another type of porin from the Gram-negative bacterium, Bordetella pertussis.Two characteristic groups of particulate proteins in oceanic waters, background proteins and specific proteins, were identified from meridional and vertical differences in their molecular mass distributions. The specific proteins were distributed over a limited range of molecular masses and one of the specific protein was identified as the heat-shock protein 70, exists in the chloroplant membrane of phytoplankton, by the N-terminal amino acid sequence analysis. Discovery of occurrences of such proteins provide invaluable information on the source and dynamics of the inanimate organic pool in the hydorsphere and global carbon cycle as well.