权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Photodynamic Therapy for Esophageal Varices using δ-Aminolevulinic acid

δ-氨基乙酰丙酸光动力疗法治疗食管静脉曲张

基本信息

批准号：
10470141
负责人：
IDO Kenichi
金额：
$ 4.35万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470141/
关键词：
esophageal varices photodynamic therapy δ-ALA HPLC レーザー血管血栓

项目摘要

Objectives : To observe the hemodynamics and histological changes of the rabbit auricles following PDT and to evaluate feasibility that PDT could be a useful adjunct to other methods for esophageal varices treatment. Methods : Photofrin (photosensitizer) and an argon-dye laser (630 nm, 300 mW/cm2) were used. Twenty rabbits were classified according to Photofrin dose : group I (2.0 mg/Kg, n : 10) ; group II (1.0 mg/Kg, n : 6) ; group III (0.2 mg/Kg, n : 4). Rabbit auricular veins were classfied according to laser illumination time : V0, no illumination ; V5, 5 min. ; V10, 10 min. ; V15, 15 min. The hemodynamics were observed with a laser Doppler blood flowmeter. The histological features of the auricles following PDT were evaluated by light microscopy. Results : The rabbits in group I and II showed a significant decrease in blood flow in V15 following PDT, but not in group III.We found a significant difference in the grade of thrombus between V5 and V15 in group I and II, and between V10 and V15 in group II.There was a significant difference in the grade of venous dilation (congestion) in V15 between group I and III (p < 0.05, Kruskal-Wallis test). Conclusions : Although further investigation is necessary to evaluate the safety and potential efficacy of phototherapy for esophageal varices, endoscopic PDT may improve outcomes beyond those achieved by any therapy alone (e.g., sclerotherapy or band ligation).

目的：观察食管静脉曲张PDT治疗后兔耳廓血流动力学和组织学变化，评价PDT辅助其他方法治疗食管静脉曲张的可行性。方法：采用Photofrin（光敏剂）和氩染料激光器（630 nm, 300 mW/cm2）。将20只家兔按Photofrin剂量分为：I组（2.0 mg/Kg, n: 10）；II组（1.0 mg/Kg, n: 6）；III组（0.2 mg/Kg, n: 4）。按激光照射时间对兔耳静脉进行分类：V0，无光照；V5, 5分钟；V10, 10分钟；V15, 15分钟。用激光多普勒血流仪观察血流动力学。光镜下观察PDT后耳廓的组织学特征。结果：PDT后，ⅰ组和ⅱ组兔V15血流量明显减少，ⅲ组无明显减少。我们发现，在I组和II组中，V5和V15之间，以及在II组中，V10和V15之间的血栓级别有显著差异。I组和III组V15静脉扩张（充血）程度差异有统计学意义（p < 0.05， Kruskal-Wallis检验）。结论：虽然需要进一步的研究来评估光疗治疗食管静脉曲张的安全性和潜在疗效，但内镜下PDT可能比任何单独治疗（如硬化治疗或绑扎）都能改善结果。