STUDY ON THE NON-INVASIVE DIAGNOSTIC SYSTEM OF HYPOXIC CELLS IN THE TUMOR BY NEAR-INFRARED SPECTROSCOPY

肿瘤缺氧细胞近红外光谱无创诊断体系的研究

• 批准号: 10470198
• 负责人: MURAYAMA Chieko
• 金额: $ 6.34万
• 依托单位: TOKAI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470198/
• 关键词: HYPOXIC CELLS; OXYGEN SATURATION; NEAR-INFRARED LIGHT; OXY-HEMOGLOBIN; DEOXY-HEMOGLOBIN; SPECT; ANTI-PIMONIDAZOLE ANTIBODY; 低酸素性細胞; 近赤外分光; 腫瘍内酸素濃度; 非侵襲的測定法; oxy-hemoglobin; deoxy-hemoglobin

THERE ARE HYPOXIC CELLS IN THE TUMOR AS A RESISTANCE FACTOR OF TREATMENT FOR A RADIATION.IT IS IMPORTANT TO KNOW THE RATE OF HYPOXIC CELLS IN THE TUMOR, THAT IS, OXYGEN CONDITION OF THE TUMOR FOR THE DECISION OF THE TREATMENT PRINCIPLE.BY THIS REASON, BEFORE AND DURING A RADIATION TREATMENT, A DEGREE OF OXYGEN SATURATION IN THE TUMOR OF PATIENTS IS MEASURED BY USING NEAR-INFRARED SPECTROSCOPY AND RELATION WITH EFFECT ON TREATMENT IS EXAMINED.AS A RESULT OF MEASURING A DEGREE OF OXYGEN SATURATION IN THE TUMOR WITH NON INVASIVE OXYGENATION MONITOR (OM-200, SHIMADZU) AND OMEGAOXYMETER (OMEGAMONITOR, BOM-L1), IT COULD SHOW A TENDENCY TO GET THE GOOD TUMOR REDUCTION RATE FOR A DEGREE OF OXYGEN SATURATION BEFORE THE TREATMENT WHICH TO BE RESPECTIVELY SUPERIOR IN 70%, 60% AND MORE.THE TUMOR THAT DEGREES OF OXYGEN SATURATION IN THE TUMOR INCREASE BY 10GY IRRADIATION (AFTER AN IRRADIATION START SEVERAL DAYS) SHOWS HIGH SENSITIVITY TOWARD THE RADIATION, AND SEEMS THAT IT CAN EXPECT AN EFFECTIVE TUMOR REDUCTION RATE.RADIO-ACTIVE COMPOUNDS WHICH ACCUMULATE INTO THE HYPOXIC CELLS WERE DEVELOPED FOR THE PURPOSE OF NUN-INVASIVE MEASURING BY SPECT.A LOTS OF FUNDAMENTAL EXPERIMENTS WERE DONE TO PREPARE FOR THE PATENT APPLICATION.FOR ANOTHER METHOD TO RECOGNIZE HYPOXIC CELLS IN THE TUMOR, PIMONIDAZOLE WHICH ALSO ACCUMULATE INTO THE HYPOXIC CELLS WAS DETECTED BY IMMUNOHISTOCHEMISTRY WITH ANTI-PIMONIDAZOLE ANTIBODY.

肿瘤中存在低氧细胞，这是放射治疗的抵抗因素。了解肿瘤中低氧细胞的比率，即肿瘤的氧状况，对于决定治疗原则是重要的。因此，在放射治疗之前和期间，用近红外线测量病人肿瘤中的氧饱和度用非侵入性氧饱和度监测仪测定肿瘤氧饱和度的红外光谱及其与疗效的关系（OM-200，岛津）和血氧饱和度测定仪（Omegamonitor，BOM-L1），在治疗前氧饱和度达到70%时，可能显示出获得良好肿瘤缩小率的趋势，60%及以上。10 Gy照射使肿瘤中氧饱和度增加的肿瘤（在辐照开始几天后）对辐射显示出高灵敏度，并且似乎它可以预期一个连续的肿瘤减少率。放射性化合物，其中积累到低氧细胞被证明是为了NUN-本研究通过大量的基础性实验，为申请专利做了大量的准备工作。

项目成果

CHIEKO MURAYAMA, ET AL: "THE EFFECT OF HYPOXIC CELL RADIOSENSITIZER IN WILD OR MUTANT P53 CELLS"NIPPON ACT RADIOL. 59. S109 (1999)

Chieko MURAYAMA 等人：“缺氧细胞放射增敏剂对野生或突变 P53 细胞的影响”NIPPON ACT RADIOL。

CHIEKO MURAYAMA, ET AL: "THE EFFECT OF HYPOXIC CELL RADIOSENSITIZER IN WILD OR MUTANT P53 CELLS"J JPN SOC THER RADIOL ONCOL. 11. 322 (1999)

Chieko MURAYAMA 等人：“缺氧细胞放射增敏剂对野生或突变 P53 细胞的影响”J JPN SOC THER RADIOL ONCOL。

CHIEKO MURAYAMA: "CLINICAL EVALUATION OF HYPOXIC CELL SENSITIZERS."JPN J CANCER CLIN. 46. 301-305 (2000)

Chieko MURAYAMA：“缺氧细胞敏化剂的临床评估。”JPN J CANCER CLIN。

Chieko Murayama, et al.: "Overcoming mutant p53 with hypoxic cell radiosensitizer,PR-350"Radiotherapy and Oncology. 56. S182 (2000)

Chieko Murayama 等人：“用缺氧细胞放射增敏剂 PR-350 克服突变型 p53”放射治疗和肿瘤学。

村山千恵子 他: "ヌードマウス移植ヒト直腸癌に対する低酸素性細胞放射線増感剤PR-350の効果の検討"日本医学放射線学会誌. 59. 897 (1999)

Chieko Murayama 等人：“低氧细胞放射增敏剂 PR-350 对裸鼠移植的人直肠癌的影响的检查”日本医学放射学会杂志 59. 897 (1999)。