STUDY ON THE NON-INVASIVE DIAGNOSTIC SYSTEM OF HYPOXIC CELLS IN THE TUMOR BY NEAR-INFRARED SPECTROSCOPY

肿瘤中有低氧细胞作为放射治疗的阻力因素。了解肿瘤中低氧细胞的比率，即肿瘤的氧气状况，对于决定治疗方案是很重要的。因此，在放射治疗前和治疗中，用近红外光谱仪测量患者肿瘤内的氧饱和度，并与治疗效果的关系。作为用非侵入性氧合监测仪(OM-200，岛津)和OMEGAOXYTER(OMEGAMONITOR，BOM-L1)测量肿瘤内氧饱和度的结果，治疗前血氧饱和度有较好的缩小率，分别为70%、60%和以上。肿瘤内血氧饱和度增加10GY照射(照射开始数天后)的肿瘤对辐射高度敏感，似乎可以期待有效的肿瘤减少率。为了进行SPECT非侵袭测量，研制了积聚在低氧细胞中的RADIO活性化合物。为专利应用做了大量的基础实验准备。用抗吡曼硝唑抗体进行免疫组织化学染色，检测其在缺氧细胞中的聚集情况。

THERE ARE HYPOXIC CELLS IN THE TUMOR AS A RESISTANCE FACTOR OF TREATMENT FOR A RADIATION.IT IS IMPORTANT TO KNOW THE RATE OF HYPOXIC CELLS IN THE TUMOR, THAT IS, OXYGEN CONDITION OF THE TUMOR FOR THE DECISION OF THE TREATMENT PRINCIPLE.BY THIS REASON, BEFORE AND DURING A RADIATION TREATMENT, A DEGREE OF OXYGEN SATURATION IN THE TUMOR OF PATIENTS IS MEASURED BY USING NEAR-INFRARED SPECTROSCOPY AND RELATION WITH EFFECT ON TREATMENT IS EXAMINED.AS A RESULT OF MEASURING A DEGREE OF OXYGEN SATURATION IN THE TUMOR WITH NON INVASIVE OXYGENATION MONITOR (OM-200, SHIMADZU) AND OMEGAOXYMETER (OMEGAMONITOR, BOM-L1), IT COULD SHOW A TENDENCY TO GET THE GOOD TUMOR REDUCTION RATE FOR A DEGREE OF OXYGEN SATURATION BEFORE THE TREATMENT WHICH TO BE RESPECTIVELY SUPERIOR IN 70%, 60% AND MORE.THE TUMOR THAT DEGREES OF OXYGEN SATURATION IN THE TUMOR INCREASE BY 10GY IRRADIATION (AFTER AN IRRADIATION START SEVERAL DAYS) SHOWS HIGH SENSITIVITY TOWARD THE RADIATION, AND SEEMS THAT IT CAN EXPECT AN EFFECTIVE TUMOR REDUCTION RATE.RADIO-ACTIVE COMPOUNDS WHICH ACCUMULATE INTO THE HYPOXIC CELLS WERE DEVELOPED FOR THE PURPOSE OF NUN-INVASIVE MEASURING BY SPECT.A LOTS OF FUNDAMENTAL EXPERIMENTS WERE DONE TO PREPARE FOR THE PATENT APPLICATION.FOR ANOTHER METHOD TO RECOGNIZE HYPOXIC CELLS IN THE TUMOR, PIMONIDAZOLE WHICH ALSO ACCUMULATE INTO THE HYPOXIC CELLS WAS DETECTED BY IMMUNOHISTOCHEMISTRY WITH ANTI-PIMONIDAZOLE ANTIBODY.