Development of knockout mouse of desmoyokin(AHNAK)and analysis of its functio

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In the present study, in order to clarify the role of the molecule (desmoyokin/AHNAK protein), we successfully established gene targetting mice (knockout mice) of desmoyokin/AHNAK protein. We screened mouse genomic DNA from mouse genomic DNA library, and prepared a targetting vector for desmoyokin/AHNAK protein. We inserted linealized targetting vector into enbryonic stem cells, and screened homologous recombinated cells. Using the homologous recombinated enbryonic stem cells, we prepare heterozygous mice and finally obtained homozygous knockout mice of desmoyokin/AHNAK protein. We have confirmed by both DNA and protein levels that these targetted mice are really lacking desmoyokin/AHNAK protein. At the same time, we also suceeded in preparation of desmoyokin/AHNAK protein knockout enbryonic stem cells, which should also be useful to examine the role of desmoyokin/AHNAK protein in the cell levels. We will examine the role of desmoyokin/AHNAK protein using these desmoyokin/AHNAK protein knockout mice and knockout enbryonic stem cells. These study should provide us with numerous important insights into the misterious giant protein, desmoyokin/AHNAK protein.