Optical Analysis of Hippocampal Network Oscillation

本研究的主要目的是了解海马体网络振荡的机制。我们计划这项研究是因为我们假设神经元树突的活跃行为和抑制性神经网络的活动之间的相互作用是神经系统信息处理的重要决定因素。为此，我们使用电压敏感染料成像技术和传统电生理技术观察和分析了海马切片制备中的神经元活动。在这项研究中，我们获得了几项重要发现。首先，我们利用电生理技术表明，海马锥体神经元上的两个独立突触输入的总和是亚线性的，而GABA介导的输入是导致亚线性的原因（Hippocampus, 2001）。其次，我们发现，通过使用电压敏感染料成像技术，亚线性求和发生在整个树突的长度上（神经科学，2002）。第三，我们发现天气诱发的树突动作电位通过使用电压敏感染料成像技术从输入位点向体细胞正交传播（神经科学，出版中）。

The main objective of the present study was to understand the mechanisms underlying network oscillation in hippocampus. We planned this study because we hypothesized that interaction between the active behavior of dendrites of neurons and the activities of inhibitory neural network is an important determinant in information processing in nervous systems. To this goal, we observed and analyzed neuronal activities in hippocampal slice preparations using both voltage-sensitive dye imaging techniques and conventional electrophysiological techniques. We have obtained several important discoveries during this study. Firstly, we have shown using electrophysiological techniques that a summation of two independent synaptic inputs on hippocampal pyramidal neurons is sublinear, and the GABA mediated inputs are responsible for the sublinearity (Hippocampus, 2001). Secondly, we found that the sublinear summation takes place throughout the entire length of the dendrites by using voltage-sensitive dye imaging techniques (Neuroscience, 2002). Thirdly, we found that synoptically evoked dendritic action potentials propagate orthoromically from the input sites towards the soma by using voltage-sensitive dye imaging techniques (Neuroscience, in press)