Optical Analysis of Hippocampal Network Oscillation

本研究的主要目的是了解海马网络振荡的机制。我们计划这项研究，因为我们假设神经元树突的活动行为和抑制性神经网络的活动之间的相互作用是神经系统中信息处理的重要决定因素。为此，我们使用电压敏感染料成像技术和常规电生理技术观察和分析海马脑片制备的神经元活动。在这次研究中，我们有了一些重要的发现。首先，我们已经使用电生理技术表明，海马锥体神经元上的两个独立突触输入的总和是次线性的，并且GABA介导的输入是次线性的原因（Hippocampus，2001）。其次，我们发现，亚线性总和发生在整个长度的树突通过使用电压敏感染料成像技术（神经科学，2002年）。第三，我们通过电压敏感染料成像技术发现天气诱发的树突状细胞动作电位从输入部位向索马正交传播（Neuroscience，出版中）。

The main objective of the present study was to understand the mechanisms underlying network oscillation in hippocampus. We planned this study because we hypothesized that interaction between the active behavior of dendrites of neurons and the activities of inhibitory neural network is an important determinant in information processing in nervous systems. To this goal, we observed and analyzed neuronal activities in hippocampal slice preparations using both voltage-sensitive dye imaging techniques and conventional electrophysiological techniques. We have obtained several important discoveries during this study. Firstly, we have shown using electrophysiological techniques that a summation of two independent synaptic inputs on hippocampal pyramidal neurons is sublinear, and the GABA mediated inputs are responsible for the sublinearity (Hippocampus, 2001). Secondly, we found that the sublinear summation takes place throughout the entire length of the dendrites by using voltage-sensitive dye imaging techniques (Neuroscience, 2002). Thirdly, we found that synoptically evoked dendritic action potentials propagate orthoromically from the input sites towards the soma by using voltage-sensitive dye imaging techniques (Neuroscience, in press)