Study on biological mechanism, specially, information transmittance by malting use of high resolution spectroscopic method

在本项目中，神奈川大学新建了一台喷嘴-喷嘴傅里叶变换微波(ETTMW)光谱仪，并对神奈川理工学院已有的FTMW进行了修改。利用这两台FTMW光谱仪，同时使用美国国家标准与技术研究所(NIST)的这台仪器，通过合作，研究了涉及大幅度运动的多肽分子和其他分子物种的纯转动光谱，以获得有助于理解生物信息传递机制的分子内相互作用信息。本项目研究的分子物种有甲基膦酸二甲酯、N-甲基丙酰胺、N-甲基甲酰胺、N-甲基乙酰胺、1，1-二甲基硅环丁烷、N-甲基苯胺、N，N-二甲基乙酰胺、芳基二甲基醚、N-二甲基醚等。例如，对于N，N-二甲基乙酰胺，通过Coriolis相互作用得到了三个CH3-内旋的转移几率，并确定了1，1-二甲基硅环丁烷分子中CH3-内旋和缩环运动相互作用的转动常数的贡献。这些量所包含的信息是有用的，它们被认为与生物相关分子的信息透过率密切相关。

In the present project, a nozzle-jet Fourier transform microwave (ETTMW) spectrometer was newly constructed in Kanazawa University, and the FTMW existing already in Kanagawa Institute of Technology was revised. Using these two FTMW spectrometers, and also using the instrument in National Institute of Standards and Technology (NIST) of U.S,A. through collaborative work, pure rotational spectra of peptide molecules and other molecular species involving large-amplitude motions were investigated to obtain information on intra-moleculaar interactions useful for understanding mechanism of biological information transmittance. Molecular species studied in the present project are dimethyl methylphosphonate, N-methylpropionamide, N-methylformamide, N-methylacetamide, 1,1-dimethylsilacyclobutane, N-methylaniline, N,N-dimethylacetamide, Ar-dimethyl ether complex, Ne-dimethyl ether complex etc. For examples, a transition probability relating to transmittanee of the three CH_3-internal rotations occurring through Coriolis interaction between overall-rotation and internal-rotation was obtained for the N,N-dimethylacetamide, and a contribution to a rotational constant of the interaction between CH_3-internal rotation and ring-puckering motion was determined for the 1,1-dimethylsilacyclobutane molecule. Information involved in these quantities is useful, which are considered to be strongly related to information transmittance of fiologically related molecules.