Regulation of the expression and translocation of reductive dehalogenases in anaerobic bacteria

多元硫螺旋体厌氧变形杆菌中有机卤化物呼吸的调节机制，与已知的细菌对环境变化的快速适应类型相反，呈现出一种新的长期机制。在这个项目中，将使用最新的基因组学和转录组学方法识别和功能表征调控网络和所涉及的组件。重点分析了四氯乙烯呼吸链生物发生的相关性和协调性。利用分子生化技术，研究细菌对PCE的检测，并阐明该信号如何转化为PCE基因的诱导表达，以及其他相关基因簇的表达、翻译和蛋白质成熟，包括蛋白质分选。该项目将揭示细菌能量代谢调节的新机制，并扩展我们目前对调节机制多样性的认识。

The regulation of the organohalide respiration in the anaerobic epsilonproteobacterium Sulfurospirillum multivoransexhibits a novel long-term mechanism which is contrary to the known types of quick adaptation of bacteria to environmental changes. In this project, the regulatory networks and the components involved will be identified and functionally characterized using up-to-date methods of genomics and transcriptomics. In particular, the correlation and the coordination of the biogenesis of the tetrachlroroethene respiratory chain will be analyzed. Using molecular-biochemical techniques, the detection of PCE by the bacterium will be investigated and the transformation of this signal into the induction of pce gene expression and that of other gene clusters involved, their translation and protein maturation including protein sorting will be elucidated. This project will unravel a novel mechanism of regulation of the bacterial energy metabolism and extend our current knowledge on the diversity of regulatory mechanisms.