High-resolution proteomic analysis of native sodium-phosphate transporters SLC34A1 and SLC34A3 and their role in kidney disease (P09)

天然钠磷酸盐转运蛋白 SLC34A1 和 SLC34A3 的高分辨率蛋白质组学分析及其在肾脏疾病中的作用 (P09)

• 批准号: 456674440
• 金额: --
• 依托单位: Lehrstuhl für Physiologie II - Molecular Physiology
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/456674440?language=en
• 关键词: resolution; proteomic; analysis; native; sodium

P9 will study the role of native transporters NaPi-IIa (SLC34A1) and NaPi-IIc (SLC34A3) by analyzing their subunit composition, operation and cell physiology in the mammalian kidney. Rare mutations in the genes encoding these proteins cause disorders of phosphate metabolism and kidney stone disease, and GWAS showed that SLC34A1 is associated with alterations in serum phosphate levels and the filtration process in kidney. P9 will use a multidisciplinary, interactive approach comprising quantitative mass spectrometry and previously developed biochemical and proteomic techniques including complexome profiling, together with functional analyses as well as fluorescence and electron microscopy to elucidate the regulation, function and interaction partners of these kidney disease targets.

P9将研究天然转运蛋白NaPi-IIa（SLC 34 A1）和NaPi-IIc（SLC 34 A3）的作用，通过分析它们在哺乳动物肾脏中的亚基组成，操作和细胞生理学。编码这些蛋白质的基因中的罕见突变导致磷酸盐代谢紊乱和肾结石疾病，GWAS表明SLC 34 A1与血清磷酸盐水平和肾脏过滤过程的改变有关。P9将使用多学科的交互式方法，包括定量质谱和先前开发的生物化学和蛋白质组学技术，包括复合体分析，以及功能分析以及荧光和电子显微镜，以阐明这些肾脏疾病靶点的调控，功能和相互作用伙伴。