Evolutionary history of ECA1 gametogenesis-related proteins and their roles in seed plant reproduction

ECA1配子发生相关蛋白的进化史及其在种子植物繁殖中的作用

• 批准号: 456687146
• 负责人: Dr. Stefanie Sprunck
• 金额: --
• 依托单位: Lehrstuhl für Zellbiologie und Pflanzenbiochemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/456687146?language=en
• 关键词: Evolutionary; history; ECA1; gametogenesis; related

Secreted small cysteine-rich proteins (CRPs) are emerging as key signaling factors regulating multiple aspects of plant vegetative and reproductive development. Striking features of plant CRPs are their evolutionary diversification in flowering plants (angiosperms) and prominence in reproductive tissues. One of the largest group of CRPs with remarkably restricted expression in the female gametophyte (embryo sac) of angiosperms is the family of ECA1 (early culture abundant 1) gametogenesis-related CRPs. In Arabidopsis thaliana, this family comprises at least 124 members but a function has only assigned for a small subfamily of five closely related proteins termed EC1 (EGG CELL 1). These egg cell-specific CRPs are only secreted cell when the two sperm cells are released from the pollen tube into the embryo sac and render the sperm cells competent to fuse with the female gametes (egg cell and central cell). This project aims to contribute to the understanding of the evolutionary history, functional diversification and differential expression of ECA1 gametogenesis-related CRPs in land plants, with a special focus on the first appearance of EC1 and EC1-related subfamilies. The project will furthermore address the functional conservation of EC1 proteins among different plant species, the EC1 posttranslational modification, and the impact of redox-dependent mechanisms on EC1 secretion and gamete fusion. Functional studies will be also performed on A. thaliana EC1-related proteins to elucidate their role(s) in reproductive development.

分泌的富含半胱氨酸的小蛋白（CRP）正在成为调节植物营养和生殖发育多个方面的关键信号因子。植物 CRP 的显着特征是它们在开花植物（被子植物）中的进化多样化以及在生殖组织中的突出地位。在被子植物雌配子体（胚囊）中表达显着受限的最大 CRP 家族之一是 ECA1（早期培养丰富 1）配子发生相关 CRP 家族。在拟南芥中，该家族至少包含 124 个成员，但功能仅分配给由 5 个密切相关的蛋白质组成的小亚家族，称为 EC1 (EGG CELL 1)。这些卵细胞特异性 CRP 仅在两个精子细胞从花粉管释放到胚囊中时才被分泌细胞，并使精子细胞能够与雌配子（卵细胞和中央细胞）融合。该项目旨在帮助了解陆地植物中ECA1配子发生相关CRP的进化历史、功能多样化和差异表达，特别关注EC1和EC1相关亚科的首次出现。该项目还将进一步解决不同植物物种之间 EC1 蛋白的功能保守、EC1 翻译后修饰以及氧化还原依赖性机制对 EC1 分泌和配子融合的影响。还将对拟南芥 EC1 相关蛋白进行功能研究，以阐明它们在生殖发育中的作用。