Functional analysis of chromatin remodeling complezes and histone modifiers

我们已经从基因调控的角度研究了核受体的功能。在本研究中，为了揭示配体诱导核受体转激活的分子基础，对核受体相关的核复合体进行了生化纯化。这些复合物包括共抑制因子、共激活因子和染色质重塑因子。其中，我们广泛研究了一种染色质重塑复合物WINAC的功能。WINAC似乎与VDR结合，通过周围核小体阵列的重组将其带到靶基因启动子处。配体结合后，其他类型的共调节复合物被招募到基因启动子中的VDR/WINAC中。我们已经从基因调控的角度研究了核受体的功能。在本研究中，为了揭示配体诱导核受体转激活的分子基础，对核受体相关的核复合体进行了生化纯化。这些复合物包括共抑制因子、共激活因子和染色质重塑因子。其中，我们广泛研究了一种染色质重塑复合物WINAC的功能。WINAC似乎与VDR结合，通过周围核小体阵列的重组将其带到靶基因启动子处。配体结合后，其他类型的共调节复合物被招募到基因启动子中的VDR/WINAC中。

We have been studied the function of nuclear receptors in terms of gene regulations. In the present study, to reveal the molecular basis of the ligand-induced transactivation of nuclear receptors, nuclear, mplexes associated with nuclear receptors have been biochemically purified. The complexes include co-repressor, co-activator and chromatin remodeling ones. Among them, we have extensively investigated the function of one of chromatin remodeling complexes, WINAC. WINAC appears to associate with VDR to bring it to the target gene promoters through reorganization of surrounding nucleosome arrays. Upon ligand binding, the other classes of co-regulator complexes are recruited to VDR/WINAC in the gene promoters.We have been studied the function of nuclear receptors in terms of gene regulations. In the present study, to reveal the molecular basis of the ligand-induced transactivation of nuclear receptors, nuclear, mplexes associated with nuclear receptors have been biochemically purified. The complexes include co-repressor, co-activator and chromatin remodeling ones. Among them, we have extensively investigated the function of one of chromatin remodeling complexes, WINAC. WINAC appears to associate with VDR to bring it to the target gene promoters through reorganization of surrounding nucleosome arrays. Upon ligand binding, the other classes of co-regulator complexes are recruited to VDR/WINAC in the gene promoters.