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Functional analysis of chromatin remodeling complezes and histone modifiers

染色质重塑复合物和组蛋白修饰物的功能分析

基本信息

批准号：
14360048
负责人：
KATO Shigeki
金额：
$ 9.02万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

We have been studied the function of nuclear receptors in terms of gene regulations. In the present study, to reveal the molecular basis of the ligand-induced transactivation of nuclear receptors, nuclear, mplexes associated with nuclear receptors have been biochemically purified. The complexes include co-repressor, co-activator and chromatin remodeling ones. Among them, we have extensively investigated the function of one of chromatin remodeling complexes, WINAC. WINAC appears to associate with VDR to bring it to the target gene promoters through reorganization of surrounding nucleosome arrays. Upon ligand binding, the other classes of co-regulator complexes are recruited to VDR/WINAC in the gene promoters.We have been studied the function of nuclear receptors in terms of gene regulations. In the present study, to reveal the molecular basis of the ligand-induced transactivation of nuclear receptors, nuclear, mplexes associated with nuclear receptors have been biochemically purified. The complexes include co-repressor, co-activator and chromatin remodeling ones. Among them, we have extensively investigated the function of one of chromatin remodeling complexes, WINAC. WINAC appears to associate with VDR to bring it to the target gene promoters through reorganization of surrounding nucleosome arrays. Upon ligand binding, the other classes of co-regulator complexes are recruited to VDR/WINAC in the gene promoters.

我们已经从基因调控的角度研究了核受体的功能。在本研究中，为了揭示配体诱导核受体反式激活的分子基础，对与核受体相关的核复合体进行了生化纯化。这些复合体包括共抑制物、共激活物和染色质重塑复合体。其中，我们对染色质重塑复合体WINAC的功能进行了广泛的研究。WINAC似乎与VDR结合，通过重组周围的核小体阵列将其带到靶基因启动子处。在与配体结合后，其他类型的协同调节复合体被招募到基因启动子中的VDR/WINAC。我们已经从基因调控的角度研究了核受体的功能。在本研究中，为了揭示配体诱导核受体反式激活的分子基础，对与核受体相关的核复合体进行了生化纯化。这些复合体包括共抑制物、共激活物和染色质重塑复合体。其中，我们对染色质重塑复合体WINAC的功能进行了广泛的研究。WINAC似乎与VDR结合，通过重组周围的核小体阵列将其带到靶基因启动子处。在与配体结合后，其他类型的协同调节复合体被招募到基因启动子中的VDR/WINAC。