Developmental analyzes of primordial germ cells and induction of germ-line chimera in fish.

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1)The origin and migration route were investigated by histological section and whole mount in situ hybridization of vas mRNA at a probe in ice goby and floating goby (Gobioidei, Perciformes, Teleostei).2)Primordial germ cells were visualized at the somitogenesis stages with GFP fluorescence in herring, goldfish, loach and ice goby embryos, when fertilized eggs of these species were injected with chimeric RNAs containing GFP coding region and zebrafish nos1 3'-untranslated region.3)A technique to visualize live PGCs using chimeric RNAs containing GFP coding region and vasa 3'-untranslated region was also established.4)When visualized PGCs at the somitogenesis stage were inter-temporally transplanted into blastoderm at the blastula stage, donor PGCs moved to genital anlage of the host embryo in con-specific transplantation.5)In the case of inter-temporal, xeno-generic transplantation, for example from goldfish to loach, donor PGCs moved to the host genital anlage through the host migration route with the host PGCs. But, in some combination of xeno-generic transplantation, such as from loach to ice goby, donor PGCs could not moved to the host genital anlage.6)A technique to generate live fry from intraperitoneally transplanted primordial germ cells was established in salmonid fish.7)A technique to isolate highly pure and viable PGCs from rainbow trout by GFP-dependent flow cytometry was established.