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Development of a vascular prosthesis coated with bioabsorbable materials free from pathological proteins

开发一种涂有不含病理蛋白的生物可吸收材料的血管假体

基本信息

批准号：
14370415
负责人：
YASUHARA Noishiki
金额：
$ 9.66万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370415/
关键词：
Blood vessel prostheses Bovine Spongious Encepharopathy collagen growth factors Ultrafine polyester fiber coated vascular prosthesis

项目摘要

A fabric vascular prosthesis coated with bioabsorbable mateiraal was developed. This project was aimed to obtain a coated fabric vascular prosthesis free from bovine spongious encepharopathy. At first, we searched a suitable material for the coating. After some examinations, we selected non-pathological protein free substances, such as cellulose, extremely high molecule hyaluronic acid, polyethylene glycol, polyvinyl alcohol, and their substrates. Our basic experiments showed that the most suitable material for this purpose was carbomethyl cellulose (CMC). Secondly, we made efforts was how to insolubilize the materials on a fabric prosthesis. We checked chemical reagents such as formaldehyde, glutarardehyde and hexamethylene diisocyanate. All of them showed cytotoxicity. After long-term washing, the materials crosslinked with these chemical reagents showed cytotoxicity. From this results, we gave up to use these cehmical reagent.Finally, we adpoted physical methos such as gannma-ray irradiation, ultraviolet irradiation., and electron beam irradiation. But all of them showed adequate damages to the fabric fibers.Therefore, we used heat-cross-linking method. This was a simple method, but it did not show any citotoxic reacton. Thirdly, we made effort to create a best matrilx of the fabric vascular prosthesis for the coating.. For this purpose, we developed a prosthesis fabricated with ultrafine polyester fibers. These ultrafine fibers can trap the coated substance very firmaly. Finally, with these basic experiments, a fabric prosthesis made of ultrafine polyester fibers which was coated with CMC. The CMC was insolubilized by heat cross-linking. We implanted the created prostheses into the thoracic descending aortae of dogs. The graft showed expected results. We are now underway to observe this in vivo experiment for long-term analysis.

研制了一种生物可吸收涂层织物人工血管。本项目旨在获得一种无牛海绵状脑病的涂层织物人工血管。首先，我们寻找一种适合做涂层的材料。经过一定的检测，我们选择了非病理性的无蛋白物质，如纤维素、极高分子透明质酸、聚乙二醇、聚乙烯醇及其底物。我们的基础实验表明，最合适的材料，用于这一目的是羧甲基纤维素（CMC）。其次，我们努力的是如何使织物修复体上的材料不溶解。我们检查了化学试剂，如甲醛，戊二醛和二异氰酸酯。所有细胞均表现出细胞毒性。经长期洗涤后，这些化学试剂交联的材料显示出细胞毒性。最后，我们采用物理方法，如γ射线照射、紫外线照射等，和电子束照射。但它们都对织物纤维有一定的损伤，因此我们采用了热交联法。这是一个简单的方法，但它没有显示任何细胞毒性反应。第三，我们努力创造一个最佳的织物人工血管涂层基体。为此，我们开发了一种用超细聚酯纤维制成的假体。这些超细纤维可以非常牢固地捕获涂层物质。最后，在这些基础实验的基础上，制备了一种由超细聚酯纤维制成的织物假体。通过热交联使CMC不溶。我们将所制造的假体植入犬的胸降椎骨中。移植物显示出预期的结果。我们现在正在进行观察这种体内实验，以进行长期分析。