Role of endocannabinoid in circulatory and nociceptive regulation systems

这项研究旨在研究大麻素激动剂和拮抗剂在体内循环和伤害性调节系统中的可能作用。为此，我们研究了大麻素激动剂和拮抗剂对炎症刺激反应中性粒细胞行为的影响。用透光显微镜观察了金黄地鼠颊囊微血管内中性粒细胞的行为。灌流Lekotrience B_4可使贴壁于内皮细胞的中性粒细胞数量增加，并在小静脉外的内皮细胞内游走。在白三烯B_4灌流前静脉注射大麻类激动剂WIN55212的金黄地鼠体内，利可三烯B_4引起的迁移明显减弱。这些作用被大麻素拮抗剂AM251和AM630消除。这些结果表明，大麻素系统的增强可以抑制中性粒细胞的迁移，有助于抑制炎性排列紊乱和痛觉过敏。

This study was designed to investigate possible roles of cannabinoid agonists and antagonists in circulatory and nociceptive regulation systems in the body. For this purpose we examined the effects of cannabinoid agonists and antagonists on neutrophil behaviors in response to inflammatory stimulation. The neutrophil behavior was observed in the microvasculature of hamster cheek pouch using a trans-illumination microscope. Superfusion of lekotrience B_4 caused an increase in the number of neutrophils adhering the endothelium and migrating through the endothelium outside the venules. The migration induced by lekotrience B_4 was significantly attenuated in hamsters receiving intravenous infusion of WIN55212, a cannabinoid agonist, prior to the leukotriene B_4 superfusion. These effects were abolished by AM251 and AM630, cannabinoid antagonists. These results suggest that augmentation of the cannabinoid system could produce an inhibition of neutrophil migration and contribute to suppression of inflammatory derangement and hyperalgesia.