Role of endocannabinoid in circulatory and nociceptive regulation systems

内源性大麻素在循环和伤害性调节系统中的作用

• 批准号: 14370494
• 负责人: HOKA Sumio
• 金额: $ 8.77万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370494/
• 关键词: cannabinoid; nociception; microcirculation; inflammation; leukocyte; migration; adhesion; pain control; 好中球; CB1受容体; CB2受容体; ロイコトリエンB_4; 血管外遊走; マリファナ; エンドトキシンショック; 白血球

This study was designed to investigate possible roles of cannabinoid agonists and antagonists in circulatory and nociceptive regulation systems in the body. For this purpose we examined the effects of cannabinoid agonists and antagonists on neutrophil behaviors in response to inflammatory stimulation. The neutrophil behavior was observed in the microvasculature of hamster cheek pouch using a trans-illumination microscope. Superfusion of lekotrience B_4 caused an increase in the number of neutrophils adhering the endothelium and migrating through the endothelium outside the venules. The migration induced by lekotrience B_4 was significantly attenuated in hamsters receiving intravenous infusion of WIN55212, a cannabinoid agonist, prior to the leukotriene B_4 superfusion. These effects were abolished by AM251 and AM630, cannabinoid antagonists. These results suggest that augmentation of the cannabinoid system could produce an inhibition of neutrophil migration and contribute to suppression of inflammatory derangement and hyperalgesia.

本研究旨在探讨大麻素激动剂和拮抗剂在体内循环和伤害性调节系统中的可能作用。为了这个目的，我们研究了大麻素激动剂和拮抗剂对中性粒细胞炎症刺激反应的影响。用透射式显微镜观察金黄地鼠颊囊微血管内中性粒细胞的行为。灌流lekotrience B_4可使粘附于内皮细胞的中性粒细胞数量增加，并可通过内皮细胞向微静脉外迁移。静脉注射大麻素激动剂WIN55212后再灌流白三烯B_4，可明显减弱白三烯B_4诱导的仓鼠迁移。这些作用被大麻素拮抗剂AM251和AM630消除。这些结果表明，大麻素系统的增强可以产生抑制中性粒细胞迁移，并有助于抑制炎症紊乱和痛觉过敏。

项目成果

Quantitative analysis of left ventricular regional wall motion with color kinesis during abdominal aortic cross-clamping.

腹主动脉阻断期间左心室区域壁运动与颜色运动的定量分析。

• 发表时间: 2003
• 期刊: J.Cardiothorac.Vasc.Anesth. 17
• 作者: Saito S;Kadoi Y;Nihishara F;Ase C;Goto F.;Naoyuki Hashiguchi;Yamaura K et al.
• 通讯作者: Yamaura K et al.

内因性カンナビノイドの役割

内源性大麻素的作用

• 发表时间: 2005
• 期刊: 臨床麻酔 29
• 作者: Fujihara;H.;外 須美夫
• 通讯作者: 外 須美夫

Migration of neutrophils elicited by leukotriene B_4 is inhibited by fasudil, a Rho-kinase inhibitor, in the microvasculature of hamster cheek pouch.

在仓鼠颊囊的微脉管系统中，Rho 激酶抑制剂法舒地尔可抑制白三烯 B_4 引起的中性粒细胞的迁移。

• 发表时间: 2004
• 期刊: Circulation Control 25
• 作者: Ozawa A et al.

The effect of dexmedetomidine on transient cerebral ischemia in rats.

右美托咪定对大鼠短暂性脑缺血的影响。

• 发表时间: 2004
• 期刊: Circ Cont 25
• 作者: Kimotsuki H;Okamoto H;Ozawa A;Hoka S
• 通讯作者: Hoka S

麻酔中の前負荷指向型循環管理

麻醉期间预负荷引导的循环管理

• 发表时间: 2003
• 期刊: 臨床麻酔 27
• 作者: Y;Tsuzuki K;Yamada N;Okado H.Miwa A;Goto F;Ozawa S.;外 須美夫
• 通讯作者: 外 須美夫