Molecular-genetical approach to mutagenesis

分子遗传学诱变方法

• 批准号: 14380251
• 负责人: TODO Takeshi
• 金额: $ 8.7万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380251/
• 关键词: UV-damage; Mutagenesis; TLS DNA polymerase; DNA damage; TLS DNAポリメラーゼ; DNA修復酵素; 突然変異生成; ショウジョウバエ; DNA修復; DNA複製酵素

Members of the Y-family of DNA polymerases catalyze template-dependent DNA synthesis but share no sequence homology with other known DNA polymerases. Y-family polymerases exhibits high error rates and low processivity when copying normal DNA but are able to synthesize DNA opposite damaged templates. We have found a novel regulatory pathway of Y-family polymerases. The Y-family polymerases are degraded by proteasome pathway in Drosophila cells when they are overexpressed. We have also identified the protein-protein interaction between Y-family polymerases. These findings will clarify the mutagenesis process in the evolutionary viewpoint.

DNA聚合酶Y家族的成员催化模板依赖性DNA合成，但与其他已知的DNA聚合酶没有序列同源性。Y家族聚合酶在复制正常DNA时表现出高错误率和低持续合成能力，但能够合成与受损模板相反的DNA。我们发现了Y家族聚合酶的一种新的调控途径。Y家族聚合酶在果蝇细胞中过表达时通过蛋白酶体途径降解。我们还鉴定了Y家族聚合酶之间的蛋白质-蛋白质相互作用。这些发现将从进化的角度阐明突变过程。

项目成果

New role of zCRY and zPER2 as regulators of sub-cellular distributions of zCLOCK and zBMAL proteins

• DOI: 10.1093/nar/gkg174
• 发表时间: 2003-02-01
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Hirayama, J;Fukuda, I;Todo, T
• 通讯作者: Todo, T

Veber, C.W.M.Kay, H.Mogling, K.Mobius, K.Hitomi, T.Todo: "Photoreactivation of the Flavin cofactor in xenopus laevis (6-4)Photolyase : Observation of a transient tyrosyl radical by time-resolved electronmagnetic resonance"Proc. Natl. Acad. Sci. USA. 99. 1

Veber、C.W.M.Kay、H.Mogling、K.Mobius、K.Hitomi、T.Todo：“爪蟾中黄素辅因子的光再激活 (6-4) 光裂解酶：通过时间分辨电子磁共振观察瞬态酪氨酰自由基”

Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy ion irradiation to diapause eggs

重离子辐照滞育卵诱导家蚕幼虫体细胞突变

• 发表时间: 2002
• 期刊: J Radiat Res 43(3)
• 作者: E.Kotani;T.Furusawa;S.Nagaoka;K.Nojima;H.Fujii;Y.Sugimura;M.Ichida;E.Suzuki;A.Nagamatsu;T.Todo;M.Ikenaga
• 通讯作者: M.Ikenaga

R.Brudler, K.Hitomi, H.Daiyasu, H.Toh, K.Kucho, M.Ishiura, M.Kanehisa, V.Roberts, T.Todo, J.Tainer, E.Getzoff: "Identification of a new cryptochrome class : structure, function and evolution"Mol Cell.. 11. 59-67 (2003)

R.Brudler、K.Hitomi、H.Daiyasu、H.Toh、K.Kucho、M.Ishiura、M.Kanehisa、V.Roberts、T.Todo、J.Tainer、E.Getzoff：“新密码色素的鉴定

Kotani E, Todo T et al.: "Somatic mutation in larvae of the silkworm, Bombyx mori, induced by heavy ion irradiation to diapause eggs"J.Radiat.Res.. 43. 193-198 (2002)

Kotani E、Todo T 等人：“家蚕幼虫中的体细胞突变，由重离子照射滞育卵诱导”J.Radiat.Res.. 43. 193-198 (2002)