Cloning of New Adipocytokine and elucidation of its pathophysiological role

新脂肪细胞因子的克隆及其病理生理作用的阐明

• 批准号: 15390288
• 负责人: SHIMOMURA Iichiro
• 金额: $ 8.9万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390288/
• 关键词: Visfatin; adipocytokine; insulin receptor; 脂肪細胞; 内臓脂肪型肥満

Fat tissue produces a variety of secreted proteins (adipocytokines) with important roles in metabolism. We isolated a newly identified adipocytokine, visfatin, that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to a protein identified previously as pre-B cell colony-enhancing factor(PBEF), a 52-kilodalton cytokine expressed in lymphocytes. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Mice heterozygous for a targeted mutation in the visfatin gene had modestly higher levels of plasma glucose relative to wild-type littermates. Visfatin binds to and activates the insulin receptor. We isolated a new adipocytokine ; Visfatin, and visfatin is a new therapeutic target of metabolic disorders such as diabetes.

脂肪组织产生多种在新陈代谢中发挥重要作用的分泌蛋白（脂肪细胞因子）。我们分离出一种新发现的脂肪细胞因子内脂素，它在人类和小鼠的内脏脂肪中高度丰富，并且其在血浆中的表达水平在肥胖的发展过程中增加。 Visfatin 对应于先前被鉴定为前 B 细胞集落增强因子 (PBEF) 的蛋白质，这是一种在淋巴细胞中表达的 52 千道尔顿细胞因子。 Visfatin 在培养细胞中发挥胰岛素模拟作用，并降低小鼠血浆葡萄糖水平。与野生型同窝小鼠相比，内脂素基因靶向突变杂合小鼠的血浆葡萄糖水平略高。内脂素结合并激活胰岛素受体。我们分离出一种新的脂肪细胞因子；内脂素，内脂素是糖尿病等代谢性疾病的新治疗靶点。

项目成果

Visfatin: a protein secreted by visceral fat that mimics the effects of insulin.

• DOI: 10.1097/01.ogx.0000172388.75302.e6
• 发表时间: 2005-08
• 期刊: Science
• 影响因子: 56.9
• 作者: A. Fukuhara;M. Matsuda;M. Nishizawa;Katsumori Segawa;Masaki Tanaka;Kae Kishimoto;Yasushi Matsuki