Non-invasive soft tissue reconstruction using therapeutic angiogenesis.

1.将bFGF基因转移到受体床可提高大鼠缺血皮瓣的存活率。在皮片抬高前2天，将含碱性成纤维细胞生长因子基因的表达载体注射到所设计的皮瓣下的背侧肌肉中，然后进行电穿孔(E-FGF组)。部分大鼠注射含LacZ基因的质粒载体(E-Cont组)作为对照。术后7d，E-FGF组的皮瓣坏死面积明显小于E-CONT组。死后血管造影术和组织学分析显示E-FGF组远端皮瓣血管生成较对照组明显增多。2.将重组bFGF可控性地输送到缺血皮瓣的受区，并将重组bFGF导入大鼠轴型皮瓣的受区，评价其治疗效果。术后即刻将碱性成纤维细胞生长因子酸性明胶水凝胶微球注射到背侧肌肉设计的皮瓣下(G-FGF组)。酸性明胶水凝胶微球是碱性成纤维细胞生长因子蛋白的控释系统。对照组大鼠以相同方式注射PBS处理的酸性明胶水凝胶微球(G-Cont组)。7天后，G-FGF组的皮瓣新生血管数明显高于G-Cont组，而G-FGF组的皮瓣坏死面积明显小于G-Cont组。3.采用聚合物胶束非病毒载体进行基因传递。然而，与单独注射裸质粒相比，聚合物胶束载体对肌肉组织的基因转移效率没有明显的优势。

1.Gene transfer of bFGF to recipient bed improves survival of ischemic skin flap.Axial skin flap was elevated in the dorsum of rat. Two days before flap elevation, expression plasmid vector containing bFGF gene was injected to the dorsal muscles beneath the designed skin flap, and then electroporation was delivered (E-FGF group). Some rats were injected with plasmid vector containing LacZ gene (E-Cont group) as control. Seven days later, the size of flap necrosis in the E-FGF group was significantly smaller than that of E-cont group. Postmortem angiograms and histological analyses showed that the vascularization in the distal part of skin flap significantly increased in the E-FGF group as compared with control.2.Controlled delivery of recombinant bFGF to recipient bed of ischemic skin flap.Recombinant bFGF was delivered to recipient bed of rat axial skin flap, and its therapeutic effect was evaluated. Immediately after flap elevation, bFGF-impregnated acidic gelatin hydrogel microspheres were injected to the dorsal muscles beneath the designed skin flap (G-FGF group). Acidic gelatin hydrogel microsphere is a controlled release system of bFGF protein. Control rats received PBS-treated acidic gelatin hydrogel microspheres in the same manner (G-Cont group). Seven days later, flap neovascularization in the G-FGF group was significantly higher than that in the G-Cont group, and then the size of flap necrosis in the G-FGF group was significantly smaller than that of the G-Cont group.3.Gene delivery with polymer micelle non-viral vector.To establish non-viral gene delivery system for recipient bed of ischemic skin flap, nano-sized polymer micelle containing plasmid DNA was evaluated. However, polymer micelle vector showed no remarkable advantage in gene transfer efficiency to muscle tissue as compared with sole injection of naked plasmid.