The elucidation of pathogenesis of developmental disorders induced by maternal nutritional condition in the fetal stage.

健康和疾病的发育起源：DOHaD范式是一个概念，在子宫经历中重新编程一个人，以应对晚年疾病的风险。在这项研究中，我们利用小鼠模型进行了实验，通过良好控制的实验程序验证了DOHaD理论在发育障碍中的有效性。AIN93G作为对照日粮，低蛋白饮食(LP)给孕前和孕期女性。出生后检测子代行为表型、脑内基因表达水平和基因启动子基因组甲基化水平。在宫内暴露于营养不良的子代在家庭笼子中的活动增加，与新对象的接触减少，社会调查减少。与AIN93G组相比，LP组成年子代表现出不同的基因表达模式和基因组甲基化模式。然而，没有检测到基因表达水平和甲基化水平之间的直接交互作用。

The Developmental Origins of Health and Disease:DOHaD paradigm is a concept that in utero experiences reprogram an individual for later-in-life risk of diseases. In this study, we conducted experiments to validate the DOHaD theory in the developmental disorder with well-controlled experimental procedures using mouse model. We provided AIN93G as control diet and low-protein diet (LP) to pre-pregnant and pregnant females. After birth, behavioral phenotypes of offspring, gene expression level in brain, and genomic methylation levels in promoters of genes were examined. The offspring which were exposed to malnutrition in utero exhibited increased activity in the home cage, decreased contact to novel object, and decreased social investigation. The adult offspring of LP group group exhibited different pattern of mRNA expression and genomic methylation pattern relative to AIN93G group. However, no direct interaction between expression levels and methylation levels of genes were detected.