Inhibition of SARS-CoV-2 by antibodies to the proteolytic cleavage sites of the spike protein

刺突蛋白蛋白水解裂解位点的抗体对 SARS-CoV-2 的抑制

• 批准号: 458682915
• 负责人: Dr. Alexander Hahn
• 金额: --
• 依托单位: Département de Chimie Organique
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/458682915?language=en
• 关键词: Inhibition; SARS; CoV; antibodies; proteolytic

Antibodies to linear epitopes of the SARS-CoV-2 spike protein have been discovered in sera from infected individuals and some of these epitopes lie next to or even encompass the proteolytic cleavage sites of the spike protein. Proteolytic processing is important for activation of the spike protein. We now want to test whether antibodies against these epitopes, which we generate through immunization of mice with the corresponding peptides, can inhibit the proteolytic activation of the spike protein, spike-mediated membrane fusion, and spike-driven infection.

SARS-CoV-2刺突蛋白的线性表位抗体已在感染者的血清中被发现，其中一些表位位于刺突蛋白的蛋白水解性切割位点附近，甚至包围着该蛋白的裂解位点。蛋白水解性处理是激活SPEKE蛋白的重要途径。我们现在想测试针对这些表位的抗体，我们通过用相应的多肽免疫小鼠而产生的抗体，是否可以抑制Spike蛋白的蛋白水解性激活，Spike介导的膜融合，以及Spike驱动的感染。