Analysis of the hydroperoxides component in oxidi zed LDL

目的研究内皮氧化脂蛋白在动脉硬化的发生中起重要作用。近年来，血浆中氧化脂蛋白的存在已被证实。然而，血浆中氧化脂蛋白的化学性质和代谢调节机制尚不清楚。本研究的目的是对与几种脂质相关疾病有关的人血清中氧化LDL的脂质成分（氢过氧化物）进行详细的定性和定量分析。结果与实验为了发展氢过氧化物的分析方法，首先采用光敏氧化法合成了胆固醇酯类（油酸、亚油酸、亚麻酸酯）的氢过氧化物。这些氢过氧化物的结构通过色谱分离成各非对映异构体后的光谱数据得到了证实。然后用上述反应合成了三酰基甘油的氢过氧化物，并采用光敏氧化法合成了三酰基甘油。利用这些合成的氢过氧化物，建立了高效液相色谱（荧光检测和化学发光检测）的分析方法，结果表明LDL组分中的过氧化物含量高于HDL组分，表明LDL组分中大部分存在脂质氢过氧化物，即氧化LDL。血脂紊乱（如胆固醇转移蛋白（CETP）缺乏和CD36缺乏）的血清中LDL和HDL部分的氢过氧化物的分析目前正在调查中，结果应在其他地方出现。本研究的目的之一是开发血清中过氧化氢的简便测定方法。，如环评法。以亚油酸胆固醇氢过氧化物为半抗原的EIA方法的发展研究也在进行中。

PurposeIt is known that oxidized lipoprotein in the endodermis is important for the generation of the arteriosclerosis. Recently, the existence of the oxidized lipoprotein in blood plasma has been confirmed. However, chemical property and metabolic regulation mechanism of the oxidized lipoprotein in blood plasma arenot sufficiently known. The purpose of the present study carries out details qualitative analysis and quantitative analysis on lipid component (hydroperoxides) of the oxidized LDL in human serum in connection with several lipid associated disorders.Results and ExperimentalsIn order to develop the analytical methods of hydroperoxides, the hydroperoxides of cholesterol esters (oleic, linoleic, linolenic acid esters) were first synthesized by the photo-sensitized oxidation. The structures of these hydroperoxides were confirmed by their spectral data after chromatographic separation into each diastereoisomeric isomer. Then hydroperoxides of triacyl glycerol are also synthesized by the above reactions, and triacy glycerols were synthesized by the photo-sensitized oxidation. Using these synthesized hydroperoxides, the analytical methods by high-performance liquid chromatography (fluorescent detection and chemiluminescent detection) were established and the present methods were peroxides in LDL fraction are higher than that of HDL fraction, showing that lipid hydroperoxides are existed in mostly LDL Fraction, i. e, oxidized LDL.The analysis of the hydroperoxides in LDL and HDL fraction of the serum from Lipid disorders, such as cholesterol transfer proten (CETP) deficiency and CD36 deficiency, are now under investigation and the results should be apperred elsewhere. One of the aime of this research is the development of convenient assay of hydroperoxide in serum., such as EIA method. The research for development of the EIA method is now also under investigation to use cholesteryl linoleate hydroperoxides as hapten.