Isolation and functional analysis of genes that regulate development and morphogenesis in rice.

本项目主要研究水稻花和叶发育调控基因的分离和分析。(1) dl位点的突变导致花的心皮向雄蕊的同质转化和叶的中脉的缺失。我们分析了dl突变体的表型和dl基因的空间表达模式，发现dl调控心皮的形成和中脉的形成。我们还发现DL与B类MADS基因具有拮抗相互作用。(2)从玉米和小麦中分离出DL同源基因，分析了它们的空间表达模式。结果表明，控制心皮形成和中脉形成的DL功能在这两种植物中都是保守的。(3) DL是YABBY基因家族的成员。从水稻中分离到7个YABBY基因，分析了YABBY蛋白的结构特征。各YABBY基因的空间表达模式与拟南芥不同，说明水稻YABBY基因的功能与拟南芥不同。(4)水稻fon1突变体花器官数量增加。这种表型似乎是由花分生组织的扩大引起的。我们试图通过定位克隆分离该基因，并发现了一个推测的FON1基因。

In this project, we studied on the isolation and analysis of genes that regulate flower and leaf development in rice.(1) The mutation occurred in the dl locus causes homeotic transformation of carpels into stamens in the flower and loss of midrib in the leaf. We analyzed phenotypes of the dl mutants and spatial expression patterns of the DL gene and revealed that DL regulates carpel specification and midrib formation. We also indicate that DL is antagonistically interacted with class B MADS genes.(2) We isolated the DL orthologs from maize and wheat and analyzed their spatial expression patterns. The result indicated that the DL functions controlling carpel specification and midrib formation are conserved in these two plants.(3) DL is a member of the YABBY gene family. We isolated seven YABBY genes from rice and analyzed structural features of YABBY proteins. Spatial expression patterns of each YABBY gene were distinct from those of the Arabidopsis YABBY genes, suggesting that the functions of rice YABBY genes are diversified from those of Arabidopsis.(4) The rice fon1 mutant have increased number of floral organs. This phenotype seems to be caused by an enlargement of floral meristem. We tried to isolate this gene by positional cloning and found a putative FON1 gene.