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Isolation and functional analysis of genes that regulate development and morphogenesis in rice.

调控水稻发育和形态发生的基因的分离和功能分析。

基本信息

批准号：
12460002
负责人：
HIRANO Hiro-yuki
金额：
$ 9.22万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460002/
关键词：
DL gene YABBY gene meristem size development and differentiation carpel floral meristem homeotic mutation rice (Oryza sativa)ホメオティク変異花トウモロコシコムギ器官決定

项目摘要

In this project, we studied on the isolation and analysis of genes that regulate flower and leaf development in rice.(1) The mutation occurred in the dl locus causes homeotic transformation of carpels into stamens in the flower and loss of midrib in the leaf. We analyzed phenotypes of the dl mutants and spatial expression patterns of the DL gene and revealed that DL regulates carpel specification and midrib formation. We also indicate that DL is antagonistically interacted with class B MADS genes.(2) We isolated the DL orthologs from maize and wheat and analyzed their spatial expression patterns. The result indicated that the DL functions controlling carpel specification and midrib formation are conserved in these two plants.(3) DL is a member of the YABBY gene family. We isolated seven YABBY genes from rice and analyzed structural features of YABBY proteins. Spatial expression patterns of each YABBY gene were distinct from those of the Arabidopsis YABBY genes, suggesting that the functions of rice YABBY genes are diversified from those of Arabidopsis.(4) The rice fon1 mutant have increased number of floral organs. This phenotype seems to be caused by an enlargement of floral meristem. We tried to isolate this gene by positional cloning and found a putative FON1 gene.

本项目主要研究水稻花叶发育调控基因的分离与分析。(1)发生在dl位点的突变导致花中心皮向雄蕊的同源异型转化和叶中脉的缺失。我们分析了DL突变体的表型和DL基因的空间表达模式，发现DL调节心皮规格和中脉的形成。我们还表明，DL是拮抗性相互作用与B类MADS基因。(2)我们从玉米和小麦中分离了DL直向同源物，并分析了它们的空间表达模式。结果表明，这两种植物中控制心皮发育和中脉形成的DL功能是保守的。(3)DL是YABBY基因家族的成员。我们从水稻中分离了7个YABBY基因，并分析了YABBY蛋白的结构特征。每个YABBY基因的空间表达模式与拟南芥YABBY基因的空间表达模式不同，表明水稻YABBY基因的功能与拟南芥的功能不同。(4)水稻fon1突变体花器官数目增加。这种表型似乎是由花分生组织的扩大引起的。我们试图通过定位克隆的方法分离该基因，并发现了一个推定的FON1基因。