Signal transduction to nucleus by phosphatidylinositol 3-kinase
Signal transduction to nucleus by phosphatidylinositol 3-kinase
批准号:
12460039
负责人:
FUKUI Yasuhisa
金额:
$9.02万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2001
中文摘要
为了了解磷脂酰肌醇3-激酶(PI3K)的信号转导,我们探讨了PIP_3结合蛋白的作用,特别是对SWAP-70蛋白的作用。我们的研究表明,SWAP-70在生长因子刺激后移动到膜皱纹上,该蛋白是RAC的鸟嘌呤核苷酸交换因子(GEE)。这种全球环境基金活性被拒绝,因为生长因子受体通过PI3K向RAC发出信号。与野生型小鼠相比,SWAP-70基因缺陷小鼠肾细胞培养物在EGF刺激后出现了膜褶皱的损伤。SWAP-70含有一个具有全球环境基金活性的水解区和一个负责PIP3结合的PH域。PH结构域与DH结构域结合以抑制全球环境基金的活性。这种与PIP_3的结合被取消,这表明PIP_3释放了PH结构域的抑制作用。
英文摘要
We have explored the roles of PIP_3 binding proteins, especially on Swap-70 protein, in order to understand the signal transduction of phosphatidylinositol 3-kinase (PI3K).Swap-70 has been identified as a ubiquitous distribution suggested its other functions. Our study revealed that Swap-70 moved to the membrane ruffles after stimulation with growth factors and that this protein was a guanine nucleotide exchange factor (GEE) for Rac. This GEF activity was refused for signaling from growth factor receptors to Rac via PI3K. Kidney cell cultures established from Swap-70 deficient mice showed impaired membrane ruffling after EGF stimulation compared with those for wild type mice.Swap-70 contains a DH domain, which exhibits the GEF activity, and a PH domain responsible for PIP3 binding. The PH domain binds to the DH domain to inhibit the GEF activity. This binding was abolished to PIP_3, suggestion that PIP_3 releases the DH domain from the inhibitory effect of the PH domain.
期刊论文(32)
专著(0)
科研奖励(0)
会议论文
Studies on regulation of actin cytoskeleton by phosphatidylinositol-3 kinase and its application.
-
批准号:14206009
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$32.95万
-
财政年份:2002
-
负责人:FUKUI Yasuhisa
-
依托单位:
Studies on signal transduction in generation and development of cancers.
-
批准号:10460037
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$8.06万
-
财政年份:1998
-
负责人:FUKUI Yasuhisa
-
依托单位:
The role of phosphoinsitides in cell responses
-
批准号:07456046
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$4.67万
-
财政年份:1995
-
负责人:FUKUI Yasuhisa
-
依托单位:
Cell growth and second messengers
-
批准号:04454071
-
项目类别:Grant-in-Aid for General Scientific Research (B)
-
资助金额:$3.01万
-
财政年份:1992
-
负责人:FUKUI Yasuhisa
-
依托单位:
海外基金