The relationship between the poly(A) tail of casein mRNA and the ageing of the mammary gland

酪蛋白mRNA多聚A尾与乳腺衰老的关系

• 批准号: 12460122
• 负责人: SAKAI Senkiti
• 金额: $ 10.37万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12460122/
• 关键词: mammary gland aging; aging and poly(A); aging and mammary function; mRNA and half-life; casein mRNA; mRNA half-life; lactation curve; マウス乳腺; 泌乳の調節機構; mRNAのポリA; 転写後調節

In mice, the mammary gland begins to synthesize milk prior to parturition. Milk production reaches a maximum at 12-13 days of lactation and is terminated at around 18 day. During lactation, mitosis does not occur. Thus, the milk-synthesizing activity of the mammary gland changes during lactation. Using the pregnant and lactating mouse mammary gland, the relationship between the poly(A) tail of casein mRNA and the ageing of the mammary gland was examined. The poly(A) tail of casein mRNA was determined by the digestion with RNase H in the presence and absence of oligo(dT). The mRNA half-life was determined by the presence of mRNA synthesis inhibitor, alfa-amanitin. The poly(A) tail length was about 50 nucleotides but elongated to about 100 nucleotides upon the onset of lactation. Approximate half-life of casein mRNA changed from 10 h to 19 h after the onset of milk synthesis. At the end of lactation, casein mRNA had the short poly(A) tail and a short half-life. The poly(A) tail shortening did not observe in the cytoplasm. Thus, we concluded that the mammary gland determined the casein mRNA stability through the change in the poly(A) tail length.

在小鼠中，乳腺在分娩前开始合成乳汁。产奶量在哺乳期的12-13天达到最大值，并在18天左右结束。在哺乳期，不发生有丝分裂。因此，在哺乳期间，乳腺的乳汁合成活动发生变化。以妊娠期和哺乳期小鼠乳腺为材料，研究了酪蛋白mRNA poly（A）尾与乳腺衰老的关系。酪蛋白mRNA的poly（A）尾通过在存在和不存在oligo（dT）的情况下用RNase H消化来确定。mRNA半衰期通过mRNA合成抑制剂α-鹅膏蕈碱的存在来确定。poly（A）尾长约50个核苷酸，但在泌乳开始时延长至约100个核苷酸。酪蛋白mRNA的近似半衰期从10小时至19小时后，牛奶合成的发病变化。在泌乳末期，酪蛋白mRNA的poly（A）尾较短，半衰期较短。胞质内未见poly（A）尾缩短现象。因此，我们得出结论，乳腺通过poly（A）尾长的变化决定酪蛋白mRNA的稳定性。

项目成果

Sun, Y.Kuraishi, T.Aoki, F.Sakai, S: "Hormonal regulation of mitochondrial Tim23 gene expression in the mouse mammary gland"Molecular and Cellular Endocrinology. 172. 177-184 (2001)

Sun，Y.Kuraishi，T.Aoki，F.Sakai，S：“小鼠乳腺中线粒体 Tim23 基因表达的激素调节”分子和细胞内分泌学。

森田琢磨, 酒井仙吉, 唐澤 豊, 近藤誠司: ""家畜"のサイエンス"文永堂出版. 189 (2002)

Takuma Morita、Senkichi Sakai、Yutaka Karasawa、Seiji Kondo：《畜牧科学》Buneido Publishing 189（2002）。

Fuchimoto D, Mizukoshi A, Sakai S, Aoki, F.: "Posttranscriptional regulation of cyclin A1 and cyclin A2 during mouse oocyte meiotic maturation and preimplantation"Developmental Biol. ogy and Reproduction. 65. 986-993 (2001)

Fuchimoto D，Mizukoshi A，Sakai S，Aoki，F.：“小鼠卵母细胞减数分裂成熟和植入前细胞周期蛋白 A1 和细胞周期蛋白 A2 的转录后调节”发育生物学。

Sun, Y. Nonobe, Kobayashi Y, E. Aoki, F. Yamamoto K, Sakai, S: "Characterization and expression of L-amino acid oxidase of mouse milk"Journal of Biological Chemistry. (in press).

Sun, Y. Nonobe, Kobayashi Y, E. Aoki, F. Yamamoto K, Sakai, S：“小鼠乳汁 L-氨基酸氧化酶的表征和表达”生物化学杂志。

Sun, Y. Kuraishi, T. Aoki, F. Sakai, S: "Hormonal regulation of mitochondrial Tim23 gene express in the mouse mammary gland"Molecular and Cellular Endocrinology. 172. 177-184 (2001)

Sun, Y. Kuraishi, T. Aoki, F. Sakai, S：“小鼠乳腺中线粒体 Tim23 基因表达的激素调节”分子和细胞内分泌学。