Construction of congenic strain of rat with abnormal NF-kB function and its molecular pathological analysis

NF-kB功能异常大鼠同类系的构建及其分子病理学分析

• 批准号: 12470051
• 负责人: MORIUCHI Tetsuya
• 金额: $ 6.4万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470051/
• 关键词: LEC rat; NF-kB; transcription; p53; glutathione peroxidase; LEC; 肝臓; GPx1

The long-Evans Cinnamon (LEC) rat is a mutant strain that develops hereditary hepatitis and spontaneous hepatocellular carcinoma. Using immortalized hepatocyte cell lines, 93C13 (LEC hepatocyte) and 93A16 (control LEA hepatocyte), we searched for a trans-acting factor lacking in the LEC rat liver. Based on our previous finding that glutathione peroxidase (GPx1) expression was consistently reduced in the LEC rat liver, we selected GPx1 gene for its analysis. Transient transfection assays of the constructs containing 5'-flanking sequences fused to the luciferase gene showed that 93C13 cells lacked factor(s) cooperating with p53 and NF-kB. These results suggested that decreased expression of GPx1 in LEC rat hepatocytes is notdue to lack of specific transcription factor but to abnormality in basic transcription factor complex.

long-Evans Cinnamon（LEC）大鼠是一种发生遗传性肝炎和自发性肝细胞癌的突变品系。使用永生化肝细胞系93 C13（LEC肝细胞）和93 A16（对照莱亚肝细胞），我们寻找LEC大鼠肝脏中缺乏的反式作用因子。基于我们以前的发现，谷胱甘肽过氧化物酶（GPx 1）的表达在LEC大鼠肝脏一致减少，我们选择GPx 1基因进行分析。含有与荧光素酶基因融合的5 ′-侧翼序列的构建体的瞬时转染测定显示93 C13细胞缺乏与p53和NF-κ B协同作用的因子。这些结果提示，LEC大鼠肝细胞GPx 1表达降低不是由于缺乏特异性转录因子，而是由于基础转录因子复合体的异常。

项目成果

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