Regulation of paramyxovirus gene expression and pathogenesis by host factors.
宿主因素对副粘病毒基因表达和发病机制的调节。
基本信息
- 批准号:12470071
- 负责人:
- 金额:$ 3.46万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Sendai virus (SeV) is a paramyxovirus causing bronchopneumonitis in rodents and possesses a nonsegmented single-stranded minus-sense RNA as a genome. In the present study we examined the function of SeV accessory V protein and the attenuation of SeV pathogenicity by egg passages to study the regulation of SeV gene expression and pathogenesis by host factors, and the following results were obtained.(1) The V protein of the Hamamatsu strain, a highly virulent field SeV isolate, has been shown to have a luxury function to facilitate virus propagation in mice but not in cultured cells as well as that of the Z strain, an egg-adapted avirulent laboratory SeV strain, and to be prominent only if virus replication was restricted. (ref. 4, 8, 14, and 17)(2) The luxuary function of the V protein has been shown to be dependent on the amino acid residues at the C terminus of the protein, which are highly conserved among paramyxoviruses, probably via protein conformation dependent on Zn binding. (ref. 1, 4, 8, and 13)(3) The V protein function, probably by coping with the host innate immunity, antagonized neither interferon action nor NK cell activity. The cellular target of the V protein remains to be clarified. (ref. 17, unpublished data)(4) Attenuation of SeV pathogenicity by egg passages has been shown to be caused at least by mutations of the nucleotides at position 20 and 24 in the leader sequence, suggesting that the leader mutaions affect virus pathogenesis by altering virus replication via interaction with host factors. (ref. 6, 12, and 14)
仙台病毒(SeV)是一种在啮齿动物中引起支气管肺炎的副粘病毒,其基因组具有非节段单链负义RNA。本研究通过检测SeV附属V蛋白的功能和SeV致病性通过卵传代的衰减来研究宿主因子对SeV基因表达和发病机制的调控,得到以下结果:(1)高毒力野外SeV分离株Hamamatsu株的V蛋白已被证明具有促进病毒在小鼠体内繁殖的功能,但在培养细胞中却没有这种功能,并且只有在病毒复制受到限制时才会突出。(参考文献4,8,14和17)(2)V蛋白的奢华功能已被证明依赖于蛋白质C端氨基酸残基,这在副粘病毒中是高度保守的,可能是通过依赖于锌结合的蛋白质构象。(参考文献1、4、8和13)(3)V蛋白的功能,可能是通过应对宿主先天免疫,既不拮抗干扰素作用,也不拮抗NK细胞活性。V蛋白的细胞靶标尚不清楚。(参考文献17,未发表的数据)(4)经卵传代的SeV致病性减弱已被证明至少是由先导序列第20和24位核苷酸突变引起的,这表明先导突变通过与宿主因子相互作用改变病毒复制,从而影响病毒的发病机制。(参考文献6、12、14)
项目成果
期刊论文数量(135)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sakaguchi, T.: "Alteration of Sendai virus morphogenesis and nucleocapsid incorporation by the mutation of cysteine residues of the matrix protein."Journal of Virology. 76. 1682-1690 (2002)
Sakaguchi, T.:“通过基质蛋白半胱氨酸残基的突变改变仙台病毒形态发生和核衣壳掺入。”病毒学杂志。
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- 影响因子:0
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Fujii, Y.: "Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA."Journal of Virology. 76. 8540-8547 (2002)
Fujii, Y.:“通过从 cDNA 中分离出致病区域,揭示了仙台病毒发病机制中前导序列的参与。”病毒学杂志。
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- 影响因子:0
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- 通讯作者:
Takemasa Sakaguchi: "Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate"Virology. 313. 581-587 (2003)
Takemasa Sakaguchi:“在高毒力野外分离株的感染模型中掩盖 V 蛋白对仙台病毒发病机制的贡献”病毒学。
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- 影响因子:0
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Huang, C.: "Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis."Journal of Virology. 74. 7834-7841 (2000)
Huang, C.:“仙台病毒 V 蛋白的锌结合能力在病毒发病机制中的参与。”病毒学杂志。
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- 影响因子:0
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Fan, X.-H.: "Emergence of anti-inflammatory monocytes in long-term surviving host of IL-10-transduced liver allografts."Cytokine. 13. 183-187 (2001)
Fan, X.-H.:“IL-10 转导的同种异体肝移植物长期存活宿主中抗炎单核细胞的出现。”细胞因子。
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YOSHIDA Tetsuya其他文献
Movement of upper extremity and kitchen knife during
上肢和菜刀的动作
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
HAYAKAWA Toru;WAKAYAMA Masafumi;KUME Masashi NAKAI Asami;YOSHIDA Tetsuya - 通讯作者:
YOSHIDA Tetsuya
Manufacturing process of Japanese "Hatakanagu" flag ornament based on human movement analysis
基于人体运动分析的日本“Hatakanagu”旗饰制作工艺
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
NISHINA Masaharu;YOSHIDA Yasuyuki;GOTO Akihiko;NAKAI Asami;YOSHIDA Tetsuya - 通讯作者:
YOSHIDA Tetsuya
Application of Machine Learning to the Particle Identification of GAPS
机器学习在 GAPS 粒子识别中的应用
- DOI:
10.2322/tastj.18.44 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
WADA Takuya;FUKE Hideyuki;SHIMIZU Yuki;YOSHIDA Tetsuya - 通讯作者:
YOSHIDA Tetsuya
Finger motion of wrapping process during "Kyogashi" making
制作“Kyogashi”时包裹过程中的手指动作
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
HAMADA Akemi;TANAKA Tatsunori;KUME Masashi;NAKAI Asami;YOSHIDA Tetsuya - 通讯作者:
YOSHIDA Tetsuya
Motion area of the "Temae" in the Way of Tea
茶道中“手前”的运动区域
- DOI:
- 发表时间:
2009 - 期刊:
- 影响因子:0
- 作者:
OTA Toru;ICHIHASHI Yuka;HAMASAKI Kanako;NAKAI Asami;YOSHIDA Tetsuya - 通讯作者:
YOSHIDA Tetsuya
YOSHIDA Tetsuya的其他文献
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{{ truncateString('YOSHIDA Tetsuya', 18)}}的其他基金
Estimation of Fatigue Strength of Adhesive Joints with High Ductility Adhesive by Simulation using Viscoplastic Constitutive Equation
使用粘塑性本构方程模拟估计高延展性粘合剂粘合接头的疲劳强度
- 批准号:
15K05742 - 财政年份:2015
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Transfer Learning based on the structure of Feature Space
基于特征空间结构的迁移学习
- 批准号:
24300049 - 财政年份:2012
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The analysis of environmental adaptability under hot conditions in the recent Japanese adults
近期日本成年人炎热条件下的环境适应性分析
- 批准号:
22300229 - 财政年份:2010
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A Clustering Method based on graph Coloring for integrating Local and Global Constraints
一种基于图着色的集成局部和全局约束的聚类方法
- 批准号:
20500123 - 财政年份:2008
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Determination of the optimal daily water intake in Japanese adults
日本成年人每日最佳饮水量的确定
- 批准号:
19500596 - 财政年份:2007
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Metaphor and meta-thinking in the modern German literature
德国现代文学中的隐喻与元思维
- 批准号:
17520139 - 财政年份:2005
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study of the Origin of the Cosmic-Ray Antiprotons using the BESS Spectrometer
使用 BESS 光谱仪研究宇宙射线反质子的起源
- 批准号:
11694104 - 财政年份:1999
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (A).
Precise Test for the Casimir Effect using Roberval's Balance
使用罗伯瓦尔天平精确测试卡西米尔效应
- 批准号:
04452020 - 财政年份:1992
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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Establishment of gene therapy using b-FGF gene-loaded Sendai virus for chronic wounds.
建立使用b-FGF基因负载仙台病毒治疗慢性伤口的基因疗法。
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Development of oncolytic sendai virus therapy for head and neck cancer for clinical application
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16K19428 - 财政年份:2016
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Oncolytic Sendai Virus-induced tumor-specific immunoresponse suppresses metastasis in animal model
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16K15726 - 财政年份:2016
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