Mechanism of acquiring angiogenesis apoptosis in urogenital cancer

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1) Genistein which is an isoflavone in soy beans and has inhibitory activity of tyrosine kinase, suppressed both vascular endothelial growth factor (VEGF) mRNA and basic fibroblast growth factor (bFGF) mRNA expression in human renal cell carcinoma (RCC) cell lines in vitro, and inhibited in vivo angiogenesis. Furthermore, genistein inhibited the cell growth in RCC cell line in a dose-dependent manner. These findings show that genistein has multiple antitumor functions, suggesting that it may be a novel therapeutic agent for RCC patients.2) Elevated levels of c-src tyrosine kinase activity due to mutation of c-src genemay result in overexpression of VEGF and cell survival in RCC. To test hypothesis, we examined c-src mutation in RCC cell lines. No mutation was detected in all cell lines, suggesting that mutation of c-src gene dose not seem to be involved in these events.3) Peroxisome proliferator-activated receptor gamma (PPAR γ) was expressed in RCC cell lines examined. PPAR γ ligand pioglitazone, a agent used in the treatment of type2 diabetes, inhibited VEGF and bFGF expression and production in RCC cells. Furthermore, it inhibited the cell growth and induced apoptosis. These findings indicate that pioglitazone may be useful in treatment RCC patients by multiple antitumor functions.