Analysis of gene of Granulicatella adiacens fibronectin-binding protein and application of the gene for clinical diagnosis.

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A gene encoding a protein with an ability of fibronectin binding was cloned from Granulicatella adiacens ATCC 49175T. DNA sequence revealed that the gene was located from 806 to 2419 on the 2540-bp EcoRI-XbaI fragment from the genomic DNA of G adiacens. The deduced amino acid sequence showed 54% identity to Lactococcus lactis 2', 3' -cyclic nucleotide 2' -phosphodiesterase (EC 3.1.4.16). An antibody specific to the recombinant protein was prepared in mice, but it was not able to bind to the cell surface of G adiacens, suggesting that the protein is not exposed on the surface of the organism. A set of PCR primers was designed according to the DNA sequences of the gene and DNA from various species were subjected to amplification. A 0.52-kb PCR product was yielded only from strains of G adiacens and closely related species produce no product. Clinical specimens of dental plaques from healthy volunteers produced a single 0.52-kb product, as did the type strain G adiacens. These results suggest that the PCR primers are applicable to detection and identification of G adiacens for clinical diagnosis.