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Establishment of a solar UV dosimeter based on the photoisomerization of (6-4)photoproducts to the Dewar isomers.

基于(6-4)光产物光异构化为杜瓦异构体的太阳紫外线剂量计的建立。

基本信息

批准号：
12558059
负责人：
NIKAIDO Osamu
金额：
$ 7.87万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We established a solar UV dosimeter measuring the UV inteiasiiy of wavelengths ranging from 300 to 340 nm.The solar UV, especially UVB(290-320nm)induces cyclobutane pyrimidine dimers(CPD)and(6-4)photoproduct(64P) in cellular DNA. Furthemore, the UV wavelengths ranging from 300 to 340 nm in solar light are known to efficiently photoisomerize 64P to its Dewar isomer(DwP). The latter is said to be highly mutagenic. Thus, we need to establish a dosimeter measuring the accumulation of the DwP in DNA for the risk assessment of solar UV. However, none of dosimeters measuring the wavelengths from 300 to 340 nm has been established so far. To establish a new UV dosimeter, we carried out the experiments nmentioned below,1) We blotted the DNA irradiated with 100 J/m^2 of UVC on a nylon membrane. The membrane was then exposed to various doses of monochromatic UV light from the Okazaki Large Spectrograph. After exposure, the membrane was treated with DEM-1 antibody immnohisitochemically and the color intensity was assayed (immuno-dot-blot : IDB). The results obtained so far revealed the photoisomerization of DwP from 64P efficiently occurs at 320 nm.2) By using the same method (IDB), we measured the UV doses from 300 to 340 nm emitted from the Mylar-filtered UVB lamp (Toshiba FL-20SE). The color intensity of the blotted DNA on the nylon membrane increased lmearly with increasing dosed of UVB. Thus, we succeeded to produce a new solar UV dosimeter measuring the wavelengths from 300 to 340 nm.

我们建立了一种太阳紫外线剂量计，测量波长范围为300至340 nm的紫外线强度。太阳紫外线，特别是UVB（290-320nm）会诱导细胞DNA中的环丁烷嘧啶二聚体（CPD）和（6-4）光产物（64P）。此外，已知太阳光中 300 至 340 nm 的 UV 波长可有效地将 64P 光异构化为其杜瓦异构体 (DwP)。据说后者具有高度致突变性。因此，我们需要建立一种剂量计来测量DNA中DwP的积累，以用于太阳紫外线的风险评估。然而，迄今为止还没有建立测量300至340 nm波长的剂量计。为了建立一种新的紫外线剂量计，我们进行了如下实验：1）我们将经100 J/m^2 UVC照射的DNA印迹到尼龙膜上。然后将膜暴露于冈崎大型光谱仪发出的不同剂量的单色紫外光下。暴露后，用 DEM-1 抗体进行免疫组织化学处理膜并测定颜色强度（免疫点印迹：IDB）。迄今为止获得的结果表明，64P 的 DwP 的光异构化在 320 nm 处有效发生。2) 通过使用相同的方法 (IDB)，我们测量了 Mylar 过滤的 UVB 灯 (Toshiba FL-20SE) 发出的 300 至 340 nm 范围内的 UV 剂量。尼龙膜上印迹 DNA 的颜色强度随着 UVB 剂量的增加而增加。因此，我们成功生产了一种新型太阳紫外线剂量计，可测量 300 至 340 nm 的波长。