The speculation of the methods how to manage the wildlife in the forests around the city.

本研究主要调查了城市森林中日本蚕的生境、习性、食性、寄生虫、副痘病毒等。2003年5月，我们在岩手大学泷泽实验森林附近捕获了一只mail亚成虫。我们在雪上安装了遥测系统，并进行了一年多的跟踪。虽然他在2004年2月之前一直呆在母亲的范围内，但后来他突然移动到北上河下游约1.5公里处，并在河沿岸的森林中建立了新的家园。事实上，我们认为沿河的森林对城市周围的野生动物来说是重要的栖息地。本文还分析了泷泽实验林中日本蚕粪便的分布情况，以说明城市森林中日本蚕选择栖息地的人为影响。采用地理信息系统（Software Arc View 3.2， ESRI）对所有粪便分布数据进行处理；计算了桩距居民区、街道、铁路、公路的距离，并生成了桩的分布方案。调查期间共发现粪堆119处，其位置特征如下：在冠层植被特征方面，与阔叶林和红松林相比，杉木等针叶林更受青睐。他们倾向于避开距离居民区和街道200米以内的地方。令人惊讶的是，与铁路的距离对粪便堆的数量影响较小。因此，我们得出结论，尽管它们居住在人类栖息地附近，但它们不喜欢与人类直接接触。通过对血清蛋白多态性的遗传检测，发现了白蛋白和转铁蛋白两个位点的变异。同时制备了一种可用于血清转铁蛋白判断的抗体。此外，本文还提出了利用粪便中提供的DNA进行雌雄区分的可能性。但它的改进是必要的，看来我们可以把它作为日本英语实地研究的工具。我们对日本血吸虫的血液进行了生化检测，但今后我们需要努力增加样本的数量，并积累基础数据。为了调查副痘病毒和奥夫病毒在日本血清中的感染情况，在原代牛睾丸细胞培养物和建立的牛细胞培养物中进行了奥夫病毒的繁殖。病毒在两种细胞培养中都能繁殖，但感染性滴度不足以制备用于血清学试验的病毒抗原。对病毒传代和克隆的进一步研究正在进行中。通过对岩手县日本血吸虫的寄生虫调查，在其皮肤中检出螨类2种、蜱类5种，在其各脏器中检出内寄生虫21种，其中蠕虫13种，原生动物寄生虫8种。该地区的crispus可能是家畜和人类某些致病性寄生虫的重要宿主

We mainly investigated the habitat use, nomerange, food habits, parasites, parapoxvirus for Japanese serows in the forest aloud the city. We captured one mail subadult serow near the Takizawa Experimantal Forest of Iwate University in May 2003. We attached telemetry system on the serow and chased it more than one year. Though he stayd his mother's range until February 2004, and then he suddenly moved about 1.5km downstream of Kitakami river, and established new homerange in the forest along the river. By the fact, we think the forests along the river are important as habitats for the wildlives around the city. We also analyzed the distribution for the feces piles of Japanese serow in Takizawa Experimental forest to demonstrate the human impact to select the habitat by Japanese serows in urban forests. We manipulated all the feces distribution data by geographic information system (Software Arc View 3.2, ESRI) ; computed the distance of the piles from residential areas, streets, and rai … More lroads and generated the distribution schemas. During the period of the survey, we found 119 feces piles in total, and the locations were characterized by the following. Regarding the characteristics of canopy vegetation, the serows preferred the coniferous needle-leaved tree forests such as cedar to broad-leaved forests and red pine forests. They tend to avoid places within 200 m from residential areas and streets. Surprisingly, the distance from the railroads had less effect on number of feces piles. Thus, we conclude that serows are not fond of direct contact with people, although they inhabit places near the habitat of people.Variation of two locus (albumin and transferin) was found by genetical examination of serum protein polymorphism. Also, we made a usuable antibody for a judgment of serum transferin. In addition, it is suggested that male and female distinction of Japanese serow is possible by DNA provided from feces. But its improvement is necessary, it seems that we can use it as tool of field study of Japanese serow. We performed the biochemical test of Japanese serow's blood, but an effort which we increase the number of examples in future, and to pile up basics data is necessary. To survey infection of parapoxvirus and orfvirus in Japanese serow, propagation of orfvirus was carried out in primary bovine testis cell cultures and established bovine cell culture. The virus propagated in both cell cultures but the infectivity titers were not enough to prepare viral antigen for serological tests. Further investigations for virus passages and cloning have been doing. By parasitic survey for the Japanese serows inhabiting Iwate Prefecture, 2 species of mites and 5 species of ticks were obtained from their skin and 21 species of endoparasites (13 of helminths and 8 of protozoan parasites) were also detected in the various internal organs. C.crispus living in this area may be important as reservoir host of some pathogenic parasites of domestic animals and humans Less