Study on reproductive system of diesel exhaust particles and on prevention of the effects by foods containing flavonoids.

本研究旨在观察柴油机尾气颗粒物（DEP）皮下染毒对婴儿精子日生产能力（DSP）、精子存活率、畸形率及睾丸、附睾精子的影响。而且，本研究的目的是：1）发现柴油机尾气颗粒物（DEP）在小鼠皮下暴露的最低剂量下，可产生阴道闭锁畸形儿（0.22mg/只和0.074mg/只）。2）研究了DEP对小鼠睾丸和附睾中每日精子产量、精子活力和精子畸形率的剂量依赖性。3）许多文献认为芳烃受体（AhR）可能与畸形的发生有关。因此，我们用四种AhR活性不同的小鼠，研究了AhR活性的差异是否与睾丸和附睾中每日精子产生率、精子存活率和精子形成率的关系。4）发现AhR活性与每日精子产生率呈负相关，精子存活率与睾丸中AhR活性呈正相关。在4种小鼠中。这些结果表明，4种小鼠的AhR与AhR活性相关。这些结果表明，AhR活性可能在精子的生成、存活和精子形态异常中起重要作用。6）在DEP依赖的AhR刺激体外系统中，研究了食物中黄酮类化合物对AhR抑制（Ah-I）的能力。抑制作用大小顺序为：keruclidine> mirystin> resuberatorol。这些类黄酮在洋葱和其他蔬菜中含量很高。

The aims of this study are to examine the changes of daily sperm production (DSP) ability, viability of sperm and malformation of infants and sperm in testis and epididymis by diesel exhaust particles (DEP) administrated subcutaneously. And, to clarify the mechanism of the abnormality by DEP and to examine the possibility for preventing the abnormality by foods were also the aims of this study.1)We found that malformation infants with vaginal atresia were born in mouse exposed subcutaneously to diesel exhaust particles (DEP) at the lowest dose of DEP (0.22mg and 0.074mg per mouse).2)we examined also dose dependency of DEP on daily sperm production, viability of sperm and malformation of sperm in testis and epididymis. Their effects were observed with upside-down U-type toDEPdose.3)Many papers suggest that arylhydrocarbon receptor (AhR) may associate with the occurrence of malformations. Therefore, we examined the relationship whether the difference of AhR activity would associate with the ratio of daily sperm production, viability of sperm and formation of sperm in testis and epididymis by using 4 species mice with different AhR activity.4)we could find the reverse relationship between AhR activity and daily sperm production ability and the positive relationship between sperm viability and AhR activity in testis.5)Furthermore, within 4 species of mice. From these results, it was suggested that AhR related with AhR activity within 4 species of mice. From these results, it was suggested that AhR activity may play an important role on sperm production, viability and the morphological abnormality of sperm.6)AhR-inhibiting (Ah-I) ability by flavonoids in foods was also examined in DEP-dependent AhR stimulation in vitro system. The inhibitory ability was ordered askerucetin> mirystin> resuberatorol. These flavonoids are contained very much in onion and other vegetables.