Development of cancer specific vector for lung carcinoma by inducing RCAS1 ligand and SCCA promoter

通过诱导RCAS1配体和SCCA启动子开发肺癌特异性载体

• 批准号: 13470265
• 负责人: MORIKAWA Toshiaki
• 金额: $ 9.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470265/
• 关键词: SCCA promoter; adenoviral vector; lung cancer; geneterapy; 肺AAH; 癌化; 遺伝子解析; caveolin-1

We made a replication-deficient recombinant adenovirus driven by the squamous cell carcinoma antigen (SCCA) promoter (Ad SCCA-KLAKLAK2). We demonstrated that the expression of KLAKLAK2 and growth inhibition were all specific to lung cancer cell lines that were SCCA positive, whereas no growth retardation was observed in human normal cell lines after Ad SCCA-KLAKLAK2 infection.Therapeutic effect was observed also in the nude mice zenograft model. Tumor growth was inhibited in the SCCA positive cell lines and side effects of the vector were not observed.

我们制备了由鳞状细胞癌抗原（SCCA）启动子驱动的复制缺陷型重组腺病毒（Ad SCCA-KLAKLAK 2）。结果表明，Ad-SCCA-KLAKLAK 2对肺癌细胞株的生长抑制和KLAKLAK 2的表达均具有特异性，而对正常人肺癌细胞株的生长无明显抑制作用，对裸鼠移植瘤模型也有一定的治疗作用。在SCCA阳性细胞系中肿瘤生长受到抑制，并且未观察到载体的副作用。

项目成果

Masato Suzuoki: "Impact of caveolin-1 expression on prognosis of pancreatic ductal adenocarcinoma"British Journal of Cancer. 87. 1140-1144 (2002)

Masato Suzuoki：“caveolin-1 表达对胰腺导管腺癌预后的影响”英国癌症杂志。

Kei Hiraoka: "Solitary fibrous tumors of the pleura : clinicopathological and immunohistochemical examination"Interactive Cardiovascular and thoracic Surgery. 2. 61-64 (2003)

Kei Hiraoka：“胸膜孤立性纤维瘤：临床病理学和免疫组织化学检查”交互式心血管和胸外科。

Kei Hiraoka: "High expression of tumor-associated antigen RCAS1 in pancreatic ductal adenocarcinoma in an unfavorable prognostic marker"International Journal of Cancer. 99. 418-423 (2002)

Kei Hiraoka：“胰腺导管腺癌中肿瘤相关抗原 RCAS1 的高表达是不利的预后标志物”国际癌症杂志。

Yoshihiro Nakakubo: "The prognostic significance of RCAS1 expression in squamous cell carcinoma of the oesophagus"Cancer Letters. 177. 101-105 (2002)

Yoshihiro Nakakubo：“食管鳞状细胞癌中 RCAS1 表达的预后意义”Cancer Letters。

Yoshihiro Nakakubo: "The prognostic significance of RCAS1 expression in squamous cell carcinoma of the oesophagus"Cancer Letters. 177. 101 (2002)

Yoshihiro Nakakubo：“食管鳞状细胞癌中 RCAS1 表达的预后意义”Cancer Letters。