Basdicmolecularmechanisms for tissue and organ formaiton: HH and FGF-dependent and regulation of positional information and compartment formation

组织和器官形成的基本分子机制：HH 和 FGF 依赖性以及位置信息和区室形成的调节

• 批准号: 13480244
• 负责人: SAIGO Kaoru
• 金额: $ 9.6万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480244/
• 关键词: Hedgehog; FGF; Branchless; Drosophila; Wingless; visceral mesoderm; Breathless; pxb; 位置情報; 区画化; Bar; ホメオボックス遺伝子; aristaless; Ras; 肢; HH; Branchless; Wingless; Breathless; トラキア; 気管; フィードバック

In the present study, we examined basic molecular mechanisms of development using compound eyes, trachea, legs and visceral mesoderm in Drosophila as model systems. In compound eyes, reiterative use of Spitz signals emanating from already differentiated ommatidial cells triggers the differentiation of around ten different types of cells. We showed evidence that the choice of cell fate by newly recruited ommatidial cells strictly depends on their developmental potential and that developmental potential formation is governed by region-specific prepattern gene expression. Spatial y and temporally regulated activity of Breathless~ranchless signaling is essential for trachea development. We showed that late btl expression is a target of Branchless/Breathless signaling and hence, Branchless/Breathless signaling contains a positive feedback loop, which may guarantee a continuous supply of fresh receptors to membranes of growing tracheal branch cells. We also showed that hi g hI evels of HH si … More gnaling activity are essential for medial-region patterning in legs. In mid-to-late third instar lag discs, high levels of HH signals repress the transcription of pxb, a newly identified gene encoding as transmembrane protein expressed specifically in the anterior compartment. Misexpression experiments indicated that Pxb may serve as a HH signaling attenuator capable of acting prior to HH-Ptc interactions, suggesting tha HH signaling in leg discs includes a pxb-repression-mediated positive feedback loop, run visceral mesoderm gives rise to circular visceral muscles. It has been demonstrated that the trunk visceral mesoderm parasegment is subdivided into at least two domains by connectin expression. We extended this by examining a greater number of visceral mesodermal genes including HH and FGF. Ectodermal HH and Wg differentially regulate the expression of the metameric targets in trunck visceral mesoderm. HH expression in PS3 appeared required for normal Dpp expression and gastric caesum formation. Branchless(FGF) in Vmw as found to serve as a guide for the initial budding of tracheal visceral branches. The metameric pattern of Vm, organized in response to ectodermal instructive signals, is thus maintained at a later time via autoregu I ation, is required for midgut morphogenesis and exerts feedback effect on trachea, ectodermal derivatives. Less

在本研究中，我们使用果蝇的复眼、气管、腿和内脏中胚层作为模型系统，研究了发育的基本分子机制。在复眼中，重复使用已分化的小眼细胞发出的 Spitz 信号会触发大约十种不同类型的细胞的分化。我们证明，新招募的小眼细胞对细胞命运的选择严格取决于它们的发育潜力，并且发育潜力的形成受区域特异性预模式基因表达的控制。 Breathless~ranchless 信号的空间和时间调节活动对于气管发育至关重要。我们发现晚期 btl 表达是 Branchless/Breathless 信号传导的目标，因此，Branchless/Breathless 信号传导包含正反馈回路，这可以保证向生长的气管分支细胞的膜持续供应新鲜受体。我们还表明，高水平的 HH si ... More 刺活动对于腿部内侧区域的模式至关重要。在第三龄滞后盘中后期，高水平的 HH 信号抑制 pxb 的转录，pxb 是一种新发现的编码跨膜蛋白的基因，在前室中特异性表达。错误表达实验表明，Pxb 可能作为 HH 信号传导衰减器，能够在 HH-Ptc 相互作用之前发挥作用，表明腿椎间盘中的 HH 信号传导包括 pxb 抑制介导的正反馈环路，运行内脏中胚层产生圆形内脏肌。已经证明，躯干内脏中胚层副段通过连接蛋白表达细分为至少两个结构域。我们通过检查更多的内脏中胚层基因（包括 HH 和 FGF）来扩展这一点。外胚层 HH 和 Wg 差异调节躯干内脏中胚层同色异体靶标的表达。 PS3 中的 HH 表达似乎是正常 Dpp 表达和胃盲肠形成所必需的。 Vmw 中的无分支（FGF）被发现可以作为气管内脏分支最初出芽的指导。 Vm 的同色异谱模式是响应外胚层指令信号而组织的，因此在以后通过自动调节得以维持，是中肠形态发生所必需的，并对气管、外胚层衍生物发挥反馈作用。较少的

项目成果

Takemae, H., Ueda, R., Okubo, R., Nakato, H., Izumi, S., Saigo, K., Nishihara, S.: "Proteoglycan UDP-galactose:β-xylose β 1.4-galacto-syltransferase I is essential for viability in Drosophila melanoagster"J. Biol. Chem.. 278. 15571-15578 (2003)

Takemae, H.、Ueda, R.、Okubo, R.、Nakato, H.、Izumi, S.、Saigo, K.、Nishihara, S.：“蛋白聚糖 UDP-半乳糖：β-木糖 β 1.4-半乳糖基转移酶I 对于黑腹果蝇的生存能力至关重要”J. Biol. Chem.. 278. 15571-15578 (2003)

Tomokazu Ohshiro: "Ligand-dependent activation of breathless FGF receptor gene in Drosophila developing trachea"Mechanisms of Development. (in press). (2002)

Tomokazu Ohshiro：“果蝇气管发育中呼吸困难 FGF 受体基因的配体依赖性激活”发育机制。

Hosono, C.: "Proteoglycan UDP-galactose : β-xylose β1,6-galactosyltrans-ferase I is essential for viability in Drosophila melanogaster"Development. 130. 41-46 (2003)

Hosono，C.：“蛋白多糖 UDP-半乳糖：β-木糖 β1,6-半乳糖基转移酶 I 对于果蝇的活力至关重要”开发。 130. 41-46 (2003)。

Hosono, C.: "Functional subdivision of trunk visceral mesoderm parasegments in Drosophila is required for gut and trachea development"Development. 130. 439-449 (2003)

Hosono, C.：“果蝇躯干内脏中胚层副节段的功能细分是肠道和气管发育所必需的”。

Takemae, H.: "Proteoglycan UDP-galactose:β-xylose β1,4-galactosyltransferase I is essential for viability in Drosophila melanogaster"J.Biol.Chem.. 278. 15571-15578 (2003)

Takemae, H.：“蛋白多糖 UDP-半乳糖：β-木糖 β1,4-半乳糖基转移酶 I 对于果蝇的活力至关重要”J.Biol.Chem.. 278. 15571-15578 (2003)