Application of Carbon Materials to Biological Treatment of Mine Drainag

碳材料在矿井排水生物处理中的应用

• 批准号: 13555275
• 负责人: SASAKI Keiko
• 金额: $ 7.49万
• 依托单位: Otaru University of Commerce
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13555275/
• 关键词: Carbon Materials; Mine Drainage; Bioremediation; Manganese-oxidizing fungus; マンガン; 生物処理

A Mn-oxidizing fungus has been isolated from Mn-deposits around the hot spring in the southern part of Hokkaido, and identified to be Phoma sp.based on morphology, physiology, and genetics. The fungus oxidizes Mn(II) ions at neutral pHs and the optimum pH was 6.8. With decrease in organic carbon sources in the mediaum, Mn(II) oxidation rate was increased. The fungal Mn(II) oxidation required peptone and yeast extract as carbon sources, and the rate were delayed by the addition of glucose. Maximum Mn(II) limit for the fungal oxidation was 120ppm, which is in the range of Mn concentrations in actual mine drainage. The PAN type of carbon fiber has clearly enhanced the fungal Mn(II) oxidation rate, and improved the transparency of the solution. The PET fiber, which has the same diameter and length, did not work similarly. This might be caused by bio-affinity of carbon fiber.The biogenic Mn-oxides were identified to be γ-MnO_2 (ramsdellite) by XRD. According to SEM observation, the biogenic Mn-oxides were porous with 4nm in an average diameter, and the pore sizes were more sharply distributed in the presence of carbon fiber. Carbon fiber might stabilize the crystal growth induced by the fungus. The porous γ-MnO_2 has the possibility for application to battery materials in addition to the second mine drainage treatment.

从北海道南部的温泉周围的Mn散布中分离出一种氧化真菌，并确定为基于形态学，生理学和遗传学的phoma sp。真菌在中性pHS处氧化Mn（II）离子，最佳pH值为6.8。随着介质中有机碳源的降低，MN（II）氧化速率增加了。真菌Mn（II）氧化需要胡椒粉和酵母提取物作为碳源，并且通过添加葡萄糖来延迟速率。真菌氧化的最大Mn（II）极限为120ppm，在实际矿山排水中的Mn浓度范围内。 PAN类型的碳纤维清楚地提高了真菌Mn（II）的氧化速率，并提高了溶液的透明度。宠物纤维的直径相同，长度相同。这可能是由碳纤维生物亲和力引起的。XRD将生物元素氧化物鉴定为γ-MNO_2（Ramsdellite）。根据SEM观察，生物元素氧化物是多孔的，平均直径为4nm，并且在存在碳纤维的情况下，孔径分布得更加明显。碳纤维可能稳定真菌诱导的晶体生长。除第二矿引流处理外，多孔γ-MNO_2还可以应用电池材料。

项目成果

K.Sasaki: "Removal of manganese ions from water by Leptothrix discophora with carbon fiber"Mater. Trans.. 43(11). 2773-2777 (2002)

K.Sasaki：“使用碳纤维的 Leptothrix discophora 从水中去除锰离子”Mater。

笹木圭子, 平島 剛, 芳賀生憲, 黒沢邦彦, 恒川昌美: "休廃止鉱山の鉱滓堆積場における鉛直コアの鉱物分布"資源処理技術. 48. 75-84 (2001)

Keiko Sasaki、Tsuyoshi Hirashima、Ikunori Haga、Kunihiko Kurosawa、Masami Tsunekawa：“封闭矿山尾矿堆中垂直岩心的矿物分布”资源加工技术。

K.Sasaki, N.Haga, T.Hirajima, K.Kurosawa, M.Tsunekawa: "Distribution and transition of heavy metals in mine tailing dumps"Mater.Trans.. 43(11). 2778-2783 (2001)

K.Sasaki、N.Haga、T.Hirajima、K.Kurosawa、M.Tsunekawa：“矿山尾矿堆中重金属的分布和转化”Mater.Trans.. 43(11)。

金野英隆, 遠藤真衣, 橘英樹, 笹木圭子: "炭素繊維によるマンガン酸化真菌の活性化-繊維であることの重要性-"炭素. (投稿中). (2004)

Hidetaka Konno、Mai Endo、Hideki Tachibana、Keiko Sasaki：“碳纤维激活锰氧化真菌 - 纤维的重要性 -”（提交中）。

K.Sasaki, T.Hirajima, N.Haga, K.Kurosawa, M.Tsunekawa: "Vertical distributions of minerals in cores at abandoned mine tailings."Resources Treatment & Technology. 48. 75-84 (2001)

K.Sasaki、T.Hirajima、N.Haga、K.Kurosawa、M.Tsunekawa：“废弃尾矿岩心中矿物质的垂直分布。”资源处理