Medical Application of New Technology for imaging second messenger system by Positron Emission Tomography

正电子发射断层扫描第二信使系统成像新技术的医学应用

• 批准号: 13557074
• 负责人: TATSUO Ido
• 金额: $ 8.7万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557074/
• 关键词: Positron Emission tomography; Radio laveled diacylglycerol; Brain Function; Tumor diagnosis; PLC activity; PLD activity; Myocardial remodeling; Recovery of injury; PLG活性評価

Clinical applications of second messenger imaging by PET has been demonstrated in the fields of brain function study, myocardial remodeling and tumor diagnosis. Basic study of cell culture and animal model made clear that the imaging by ^<11>C-labeled 1,2-diacylglycerol ([^<11>C]DAG) response to PLC activity and ^<18>F-labeled 1,2-diacylglycerol ([^<18>F]FDAG) response to PLD activity. [^<11>C]DAG is used as potential imaging tracers for postsynaptic biological responses in human brain of healthy normal subjects and with various diseases such as stroke, Parkinson's and Alzheimer's disease. The [^<11>C]DAG study in myocardial infarction(MI) suggested that this technology may be useful for visually detecting regions with activated PI metabolism in the process of ventricular remodeling after MI.[^<18>F]FDAG applied to tumor detection based on the response to PLD activity in cultured cell, tumor tissues and animal models and the results suggested that [^<18>F]FDAG are used as potential imaging tracers for early stage of tumor growth.The simplified PET-MR fusion imaging computer program has been developed to promote clinical application of kinetic analysis.This technology for medical imaging of second messenger systems will be new frontier in various fields of rmedical diagnosis.

PET第二信使成像已在脑功能研究、心肌重塑和肿瘤诊断等领域得到临床应用。细胞培养和动物模型的基础研究表明，11&gt；C标记的1，2-二酰甘油([^&lt；11&gt；C]DAG)对PLC活性的显影和^&lt；18&gt；F标记的1，2-甘油([^&lt；18&gt；F]FDAG)对PLD活性的显影。[^&lt；11&gt；C]DAG被用来作为潜在的成像示踪剂，用于研究健康正常人以及中风、帕金森和阿尔茨海默病等各种疾病的人脑突触后生物学反应。对心肌梗死(MI)的DAG研究表明，这项技术可能有助于直观地检测MI后心室重构过程中PI代谢激活的区域。FDAG应用于肿瘤检测，是基于培养细胞、肿瘤组织和动物模型对PLD活性的反应，结果表明[^&lt；18&gt；FDAG是肿瘤生长早期潜在的影像示踪剂。简化的PET-MR融合成像计算机程序已被开发出来，以促进动力学分析的临床应用。这项第二信使系统的医学成像技术将成为各种医学诊断领域的新前沿。

项目成果

M.Chida, Y.Kagaya, S.Nagata, M.Mukoyoshi, S.Namiuchi, Y.Yamane, N.Ishide, J.Watanabe, T.Ido, et al.: "[^<18>F] labeled diacylglycerol analogueas a potential agent to trace myocardial phosphoinositide metabolism"Nuclear Medicine and Bioloby. 28. 815-819 (2

M.Chida、Y.Kagaya、S.Nagata、M.Mukoyoshi、S.Namiuchi、Y.Yamane、N.Ishide、J.Watanabe、T.Ido 等人：“[^18F] 标记的二酰基甘油

今堀良夫, 辻野仁, 笹島浩泰, 峯浦一喜, 近藤正樹: "パーキンソン症候群における脳内イノシトールリン脂質代謝のPET測定--運動負荷によるpallidothalamic systemの活性化--"Progress in Medicine. 22・11. 2919-2923 (2002)

Yoshio Imahori、Hitoshi Tsujino、Hiroyasu Sasashima、Kazuki Mineura、Masaki Kondo：“帕金森综合征中脑肌醇磷脂代谢的 PET 测量——运动负荷激活苍白丘脑系统——”医学进展 22. 11. 2919-2923（ 2002）

Imahori, Y, Fujii R, Tujino H, Kimura M, Mineura K.: "Positron emission tomography : measurement of the activity of second messenger systems"Methods. 27. 251-262 (2002)

Imahori, Y, Fujii R, Tujino H, Kimura M, Mineura K.：“正电子发射断层扫描：第二信使系统活性的测量”方法。

Kagaya Y, Chida M, Iwhori Y, Fujii R, Namiuchi S, Takeda M, Yamane Y, Otani H, Watanabe J, Fukuchi M, Tezuka F, Ido T, Shirato K.: "Effect of angiotensin converting enzyme inhibition on myocardial phosphoinositide metabolism Visualized with 1-[1-11C]-buty

Kagaya Y、Chida M、Iwhori Y、Fujii R、Namiuchi S、Takeda M、Yamane Y、Otani H、Watanabe J、Fukuchi M、Tezuka F、Ido T、Shirato K.：“血管紧张素转换酶抑制对心肌磷酸肌醇的影响

Chida M, Kagaya Y. Nagata S, Mukoyoshi M, Namiuchi S, Yamane Y, Ishide N, Watanabe J, Takahashi T, Ido T, Shirato K.: "[18F] labeled diacylglycerol analogue as a potential agent to trace myocardial phosphoinositide metabolism"Nucl. Med. Biol.. 28. 815-819

Chida M，Kagaya Y. Nagata S，Mukoyoshi M，Namiuchi S，Yamane Y，Ishide N，Watanabe J，Takahashi T，Ido T，Shirato K.：“[18F]标记的二酰甘油类似物作为追踪心肌磷酸肌醇代谢的潜在试剂