Application of a ciliate, Paramecium, as a sensor for a chemical substances

纤毛虫草履虫作为化学物质传感器的应用

• 批准号: 13558074
• 负责人: IWADATE Yoshiaki
• 金额: $ 2.24万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558074/
• 关键词: Paramecium; Calcium; cell; cilia

Ciliate have characteristic cell functions, such as ciliary reversal, contraction of cell body, exocytosis and so on. These cell functions are induced by a contact of several stimuli such as chemical substances. It is known that these cell functions are controlled by intracellular calcium level.In this study, we investigate the relationship between the cell functions and intracellular calcium level in order to apply ciliates as a sensor for chemical substances.In response to the application of picric acid to the cell surface of a Paramecium caudatum cell, the Paramecium discharged trichocysts through the long term calcium transient (>3 s). In the case when ethanol was applied, the Paramecium discharged trichocysts. In this case, the calcium transient was short (<1 s). These results demonstrate that the intracellular calcium behavior is depending on the chemical stimuli.Next, we injected the fluorescent calcium indicator Calcium Green into a ciliate Didiniun nasutum and observed the intra-ciliary calcium level during spontaneous ciliary reversal of the cirri. In D. nasutum, calcium rose throughout the length of the cilia accompanied by ciliary reversal. These results strongly suggest that calcium influx sites for ciliary reversal are distributed throughout the cilium.

纤毛虫具有独特的细胞功能，如纤毛翻转、胞体收缩、胞吐等。这些细胞功能是由化学物质等几种刺激物的接触引起的。这些细胞功能是由细胞内钙水平控制的。在本研究中，我们研究了细胞功能和细胞内钙水平之间的关系，以便将纤毛虫作为化学物质的传感器。在尾草履虫细胞表面施加苦味酸后，草履虫通过长期钙瞬变释放毛囊(&gt；3 S)。在使用乙醇的情况下，草履虫排出毛囊。在这种情况下，钙瞬变是短的(&lt；1 S)。这些结果表明，细胞内钙的行为依赖于化学刺激。接下来，我们将荧光钙指示剂钙绿注射到纤毛虫的鼻腔内，并观察了在自发的睫状体翻转过程中纤毛内的钙水平。在鼻侧丝虫中，钙离子在纤毛的整个长度上升高，并伴随着纤毛的反转。这些结果强烈地表明纤毛翻转的钙内流部位分布在纤毛各处。